Redesigning the Architecture of the Base Pair: Toward Biochemical and Biological Function of New Genetic Sets
Redesigning the Architecture of the Base Pair: Toward Biochemical and Biological Function of New Genetic Sets
复制标题
DOI：
10.1016/j.chembiol.2008.12.004
复制
发表时间：
2009-03-27
影响因子：
--
通讯作者：
Kool, Eric T.
中科院分区：
文献类型：
--
作者：
Krueger, Andrew T.;Kool, Eric T.
DNA支架内核酸碱基的识别包括遗传信息的传递、决定蛋白质和细胞组装、生物体发育和进化的基础。部分由于需要测试我们目前对这种信息传递的理解，化学家已经开始设计和合成非天然碱基和碱基对结构，以模拟DNA的功能，而不依赖于自然界的嘌呤-嘧啶碱基对支架。最近已经描述了多个实例，其在体外稳定地自组装并且序列特异性，并且一些分离的非天然碱基对也可以在体外复制。此外，最近在细菌细胞中使用非天然碱基的实验已经证明了化学信息的惊人有效阅读。这表明未来有可能重新设计和替换进化细胞的化学信息，同时保留生物功能。
Recognition of the nucleic acid bases within the DNA scaffold comprises the basis for transmission of genetic information, dictating protein and cell assembly, organismal development, and evolution. Driven in part by the need to test our current understanding of this information transfer, chemists have begun to design and synthesize nonnatural bases and base pair structures to mimic the function of DNA without relying on Nature's purine-pyrimidine base pair scaffold. Multiple examples have been recently described that self-assemble stably and sequence specifically in vitro, and some isolated unnatural base pairs can be replicated in vitro as well. Moreover, recent experiments with unnatural bases in bacterial cells have demonstrated surprisingly efficient reading of the chemical information. This suggests the future possibility of redesigning and replacing the chemical information of an evolving cell while retaining biological function.