Zinc uptake system (znuA locus) of Brucella abortus is essential for intracellular survival and virulence in mice
Zinc uptake system (znuA locus) of Brucella abortus is essential for intracellular survival and virulence in mice
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DOI：
10.1292/jvms.66.1059
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发表时间：
2004-09-01
影响因子：
1.2
通讯作者：
Watari, M
中科院分区：
文献类型：
--
作者：
Kim, S;Watanabe, K;Watari, M
布鲁氏菌是兼性细胞内病原体，能够在专职和非专职吞噬细胞中存活和繁殖，并导致家畜流产和人类波浪热。其致病机制和致病因素尚未完全清楚。高亲和性锌摄取系统蛋白突变体（znuA突变体）在锌螯合培养基中的生长减少，并且在HeLa细胞和小鼠骨髓源性巨噬细胞中不能复制。用含有znuA基因的穿梭载体pBBR 1 MCS-4转化znuA突变体，使其在锌螯合培养基中的生长以及在HeLa细胞和巨噬细胞中的细胞内复制恢复到与野生型菌株相当的水平。细菌内化到HeLa细胞和巨噬细胞中，并与znuA突变体的晚期内体或溶酶体共定位与野生型菌株没有不同。这些结果表明，znuA不有助于B的细胞内运输。流产，但有助于利用细胞内生长所需的锌。
Brucella spp. are facultative intracellular pathogens that have the ability to survive and multiply in professional and nonprofessional phagocytes, and cause abortion in domestic animals and undulant fever in humans. The mechanism and factors of virulence are not fully understood. High-affinity zinc uptake system protein mutant (znuA mutant) showed reduced growth in zinc chelated medium, and failed to replicate in HeLa cells and mouse bone marrow-derived macrophages. Transformation of znuA mutant with a shuttle vector pBBR1MCS-4 containing znuA gene restored the growth in zinc chelated medium and intracellular replication in HeLa cells and macrophages to a level comparable to that of wild-type strain. Bacterial internalization into HeLa cells and macrophages and co-localization with either late endosomes or lysosomes of znuA mutant were not different from those of wild-type strain. These results suggest that znuA does not contribute to intracellular trafficking of B. abortus, but contributes to utilization of zinc required for intracellular growth.