Commercially available histoplasmin sensitized latex particles in an agglutination test for histoplasmosis
Commercially available histoplasmin sensitized latex particles in an agglutination test for histoplasmosis
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DOI：
10.1007/bf02051586
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发表时间：
1962-01-01
期刊：
影响因子：
--
通讯作者：
CAMPBELL, CHARLOTTE C.
中科院分区：
文献类型：
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作者：
HILL, GRACE B.;CAMPBELL, CHARLOTTE C.
本实验室的早期研究表明，火棉胶凝集试验在区分原发性组织胞浆菌病与细菌性或病毒性肺炎方面特别有价值。然而，生产均匀批次的颗粒是测试再现性的严重障碍，并且颗粒必须在测试中使用之前每天进行敏化。最近，一种组织胞浆菌素致敏乳胶颗粒的商业制剂已经出现，它在盐水中稀释后，可以直接加入血清稀释液中。在目前的研究中，该制剂与50例组织胞浆菌病在感染的不同阶段的血清进行了测试。与本实验室制备和敏化的最好的火棉胶颗粒所获得的反应相比，反应是有利的。血清从某些类型的组织胞浆菌病是阴性的，这些也被描述。有了这种抗原，每个实验室就可以立即进行组织胞浆菌病的凝集试验，从而增加在急性期、初期阶段确诊的病例数，并消除目前因将血清运送到其他更专业的实验室而必须延迟获得结果的情况。|| 摘要编辑：作者
Earlier studies in this laboratory revealed that the collodion agglutination test was especially valuable in differentiating primary histoplasmosis from bacterial or viral pneumonias. The production of uniform lots of particles, however, was a serious handicap in the test's reproducibility, and particles had to be sensitized daily just prior to use in the test. Recently, a commercial preparation of histoplasmin sensitized latex particles became available which after dilution in saline as directed could be added directly to dilutions of sera. In the current studies this preparation was tested with sera from 50 cases of histoplasmosis in varying stages of the infection. Reactions compared favorably with those obtained with the best lots of collodion particles prepared and sensitized in this laboratory. Sera from certain types of histoplasmosis were negative and these are also described. The availability of such an antigen should make the agglutination test for histoplasmosis immediately accessible to every laboratory, thereby increasing the number of cases definitively diagnosed during the acute, primary phase and eliminating the delay in obtaining results currently necessitated by the shipping of sera to other more highly specialized laboratories. || ABSTRACT AUTHORS: Authors