Evidence of complex transcriptional, translational, and posttranslational regulation of the extracytoplasmic function sigma factor σE in Mycobacterium tuberculosis
Evidence of complex transcriptional, translational, and posttranslational regulation of the extracytoplasmic function sigma factor σE in Mycobacterium tuberculosis
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DOI：
10.1128/jb.00622-08
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发表时间：
2008-09-01
影响因子：
3.2
通讯作者：
Provvedi, Roberta
中科院分区：
文献类型：
--
作者：
Dona, Valentina;Rodrigue, Sebastien;Provvedi, Roberta
细胞外因子（ECF）σ因子σ（E）是结核分枝杆菌中研究最多的σ因子之一。它已被证明是参与毒力以及在高温，碱性pH值的条件下，暴露于洗涤剂和氧化应激的生存。与许多ECF sigma因子不同，sigma（E）不直接调节自身基因的转录。在sigE基因的上游已经鉴定出两个启动子;一个由双组分系统MprAB调节，而另一个已经显示出是sigma（H）依赖性的。在本文中，我们通过鉴定其反sigma因子和先前未知的启动子来进一步表征sigma（E）的调控。最后，我们表明，sigE可以从三个不同的翻译起始密码子翻译，这取决于所使用的启动子。总之，我们的数据表明，sigma（E）不仅受到复杂的转录调控，但也控制在翻译和翻译后水平。
The extracytoplasmic factor (ECF) sigma factor sigma(E) is one of the most studied sigma factors of Mycobacterium tuberculosis. It has been shown to be involved in virulence as well as in survival under conditions of high temperature, alkaline pH, and exposure to detergents and oxidative stress. Unlike many ECF sigma factors, sigma(E) does not directly regulate the transcription of its own gene. Two promoters have been identified upstream of the sigE gene; one is regulated by the two-component system MprAB, while the other has been shown to be sigma(H) dependent. In this paper, we further characterize the regulation of sigma(E) by identifying its anti-sigma factor and a previously unknown promoter. Finally, we show that sigE can be translated from three different translational start codons, depending on the promoter used. Taken together, our data demonstrate that sigma(E) not only is subjected to complex transcriptional regulation but is also controlled at the translational and posttranslational levels.