Zearalenone interferes with the sperm-triggered inflammation in the bovine uterus in vitro: Negative impact on sperm motility and survival
Zearalenone interferes with the sperm-triggered inflammation in the bovine uterus in vitro: Negative impact on sperm motility and survival
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DOI：
10.1016/j.reprotox.2021.12.001
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发表时间：
2021-12-07
影响因子：
3.3
通讯作者：
Miyamoto, Akio
中科院分区：
文献类型：
--
作者：
Elweza, Ahmed E.;Marey, Mohamed A.;Miyamoto, Akio
玉米赤霉烯酮（ZEN）污染的饲料对牛的繁殖产生不利影响。最近，我们报道了活跃的精子在体外诱导牛子宫内膜上皮细胞（BEEC）的促炎反应。本研究旨在研究ZEN的存在对精子-子宫串扰的影响。用ZEN（10、100和1000 ng/mL）刺激BEEC单层0、3、6、12或24 h，并通过实时PCR分析基因表达。此外，BEEC预暴露于ZEN（10，100和1000 ng/mL）24 h，然后与精子共孵育6 h。在预暴露于ZEN后，收获来自精子-BEEC共培养物的条件培养基（CM），并用于挑战多形核细胞（PMN）或精子。然后评估PMNs对精子的吞噬活性和精子活力参数。结果显示，单独的ZEN通过在不同时间点诱导促炎细胞因子（TNFA和IL 1B）和PGES 1的mRNA表达来诱导BEEC中的促炎反应。BEEC预暴露于ZEN，放大了BEEC中促炎细胞因子（TNFA和IL 1B）和趋化因子IL 8 mRNA丰度的精子触发上调。精子BEECs条件培养基，引发ZEN，刺激中性粒细胞吞噬精子，而抑制精子运动参数。综上所述，这些发现表明ZEN的存在增强了BEEC中精子引发的促炎级联反应，引起了PMNs对精子的吞噬作用，并降低了精子活力参数。这种免疫反应可能会在牛子宫内为精子的能力和存活创造一个不利的环境，从而损害生育力。
Zearalenone (ZEN)-contaminated diets induce detrimental effects on the bovine reproduction. Recently, we reported that active sperm induce pro-inflammatory responses in bovine endometrial epithelial cells (BEECs) in vitro. This study aimed to investigate the impact of presence of ZEN on the sperm-uterine crosstalk in vitro. BEECs monolayers were stimulated by ZEN (10, 100, and 1000 ng/mL) for 0, 3, 6, 12, or 24 h and gene expressions were analyzed by real-time PCR. Moreover, BEECs were pre-exposed to ZEN (10, 100, and 1000 ng/mL) for 24 h then, co-incubated with sperm for 6 h. Conditioned media (CM) from a sperm-BEECs co-culture, after pre exposure to ZEN, were harvested and exploited to challenge either polymorphonuclear cells (PMNs) or sperm. Both PMNs phagocytic activity toward sperm and sperm motility parameters were then assessed. Results showed that ZEN alone induced pro-inflammatory responses in BEECs through the induction of mRNA expressions of pro inflammatory cytokines (TNFA and IL1B) and PGES1 at different time points. Pre-exposure of BEECs to ZEN, amplified the sperm-triggered upregulation of pro-inflammatory cytokines (TNFA and IL1B) and chemokine IL8 mRNA abundance in BEECs. Sperm-BEECs conditioned media, primed by ZEN, stimulated the PMNs phagocytosis for sperm whereas suppressed sperm motility parameters. Taken together, these findings indicate that the presence of ZEN augments the pro-inflammatory cascade triggered by sperm in BEECs, provokes PMNs phagocytosis for sperm, and reduces sperm motility parameters. Such immunological reactions may create a hostile environment for sperm competence and survival in the bovine uterus, thus impair fertility.