Characterization of long non-coding RNAs involved in cadmium toxic response in Brassica napus
Characterization of long non-coding RNAs involved in cadmium toxic response in Brassica napus
复制标题
甘蓝型油菜镉毒性反应中涉及的长非编码 RNA 的表征
DOI：
10.1039/c6ra05459e
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发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Yang, Zhi Min
中科院分区：
文献类型：
--
作者：
Feng, Sheng Jun;Zhang, Xian Duo;Yang, Zhi Min
越来越多的证据表明，长链非编码RNA（lncRNA）参与了对环境胁迫的各种生物反应。有毒重金属镉（Cd）是环境中的主要无机污染物之一。lncRNA如何参与双子叶植物对镉胁迫的响应及其调控机制尚不清楚。本研究采用链特异性RNA测序（RNA-Seq）技术对镉胁迫下油菜（Brassica napus）的lncRNA进行了分析。对应于5038个唯一B的超过6000万个干净读段。鉴定了油菜lncRNA，其中2546个lncRNA由有义链产生，2492个由天然反义转录本（lncNATs）产生。组合分析鉴定了301个响应Cd胁迫的lncRNA。镉诱导的lncRNA的表达似乎与其附近的蛋白质编码基因。lncRNA基因座两侧的许多编码基因参与了镉的吸收、转运和胁迫反应，表明相关的lncRNA可能具有类似的功能。四种lncRNA被鉴定为miR 824、miR 167 d、miR 156 d和miR 156 e的前体。67个lncRNA作为竞争的内源性靶标模拟物（eTM）的36个miRNA可能参与镉胁迫反应。通过将三种lncRNA瞬时转化到B中来验证功能模拟物。在双35 S启动子和诱饵lncRNA（d35 S-lncRNA-pAN 580）的控制下的napus原生质体。这些结果表明，一组推定的lncRNA参与响应镉胁迫在B。油菜。
There is increasing evidence of long non-coding RNA (lncRNA) involvement in a variety of biological responses to environmental stresses. The toxic heavy metal cadmium (Cd) is one of the major inorganic contaminants in environments. How lncRNAs are involved in Cd stress-induced response and regulatory mechanism in dicot plants is unknown. The present study performed strand-specific RNA-sequencing (RNA-Seq) to profile lncRNAs in Cd-exposed rapeseed (Brassica napus). More than 60 million clean reads corresponding to 5038 unique B. napus lncRNAs were identified, in which 2546 lncRNAs were generated from the sense strand and 2492 were generated from natural antisense transcripts (lncNATs). A combinational analysis resulted in identification of 301 lncRNAs responding to Cd stress. Cd-induced expression of lncRNAs appeared to be associated with their nearby protein-coding genes. Many coding genes flanking lncRNA loci were involved in Cd uptake, translocation and stress-responses, suggesting that the associated lncRNAs might have similar functions. Four lncRNAs were identified as precursors of miR824, miR167d, miR156d and miR156e. Sixty-seven lncRNAs acted as competing endogenous target mimics (eTMs) for 36 miRNAs potentially involved in Cd stress response. The functional mimics were validated by transient transformation of three lncRNAs into B. napus protoplasts under control of double 35S promoter with a decoy lncRNA (d35S-lncRNAs-pAN580). These results suggest that a set of putative lncRNAs were involved in response to Cd stress in B. napus.