Study on the binding of base-mismatched oligonucleotide d(GCGAGC)2 by cobalt(III) complexes
Study on the binding of base-mismatched oligonucleotide d(GCGAGC)2 by cobalt(III) complexes
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DOI：
10.1002/ejic.200401041
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发表时间：
2005-08-05
影响因子：
2.3
通讯作者：
Pu, XH
中科院分区：
文献类型：
--
作者：
Chen, HL;Yang, P;Pu, XH
首次通过核磁共振波谱研究了 [Co(phen)(2)(HPIP)]Cl-3 和 [Co(phen)(2)(DPQ)]Cl-3 与含有两个剪切 G:A 错配的寡核苷酸 d(GCGAGC)(2) 的结合。对于这两种复合物，在钴复合物结合的六核苷酸的 NOESY 光谱中观察到相当数量的分子间 NOE。结果表明，[Co(phen)(2)(HPIP)]Cl-3与HPTP一起从小沟插入G(3):A(4)碱基对之间并延伸至大沟，并且[Co(phen)(2)(DPQ)](3+)从两个方向结合到末端G(5):C-6区域。 P-31 NMR谱表明，[Co(phen)(2)(HPIP)]Cl-3结合会引起错配碱基对区域磷酸酯主链的变化，而[Co(phen)(2)(DPQ)](3+)不会引起主链的明显变化。熔化实验表明[Co(phen)(2)(HPIP)]Cl-3 稳定双链结构，而[Co(phen)2(DPQ)]Cl-3 导致双链解旋。 (c) Wiley-VCH Verlag GmbH & Co. KGaA，69451 Weinheim，德国，2005 年。
The binding of [Co(phen)(2)(HPIP)]Cl-3 and [Co(phen)(2)(DPQ)]Cl-3 to the oligonucleotide d(GCGAGC)(2) containing two sheared G:A mispairs has been studied by NMR spectroscopy for the first time. For both the complexes, a considerable number of intermolecular NOEs were observed in NOESY spectra of the cobalt-complex-bound hexanucleotide. The results suggest that [Co(phen)(2)(HPIP)]Cl-3, with HPTP, intercalates between the G(3):A(4) base pairs from the minor groove and extends to the major groove, and [Co(phen)(2)(DPQ)](3+) binds to the terminal G(5):C-6 region from two directions. P-31 NMR spectroscopy indicates that [Co(phen)(2)(HPIP)]Cl-3 binding induces a change in the phosphate backbone in the region of the mismatched base pairs, while [Co(phen)(2)(DPQ)](3+) does not cause obvious changes in the backbone. Melting experiments indicate that [Co(phen)(2)(HPIP)]Cl-3 stabilizes the double-strand structure, while [Co(phen)2(DPQ)]Cl-3 cause the duplex to untwist. (c) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005.