Non-transferrin-bound iron is associated with biomarkers of oxidative stress, inflammation and endothelial dysfunction in type 2 diabetes
Non-transferrin-bound iron is associated with biomarkers of oxidative stress, inflammation and endothelial dysfunction in type 2 diabetes
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DOI：
10.1016/j.jdiacomp.2015.05.017
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发表时间：
2015-09-01
影响因子：
3
通讯作者：
Pinkney, Jonathan H.
中科院分区：
文献类型：
--
作者：
Aljwaid, Husam;White, Desley L.;Pinkney, Jonathan H.
目的：探讨不同程度肥胖的 2 型糖尿病受试者和非糖尿病受试者中循环非转铁蛋白结合铁 [NTBI] 与氧化应激、内皮功能和炎症标志物之间的关联。方法：通过 HPLC 测量血浆 NTBI、总铁、铁结合力、转铁蛋白饱和度和可溶性转铁蛋白受体，以及总和还原型抗坏血酸、丙二醛 [MDA]、E-选择素和对 28 名 2 型糖尿病受试者、28 名非肥胖对照者和 17 名肥胖非糖尿病受试者进行高敏 C 反应蛋白 [hs-CRP] 检测。结果：糖尿病组 NTBI 水平高于对照者，但血清总铁水平较低。糖尿病组和肥胖组的 MDA 水平均高于对照组，这与氧化抗坏血酸水平较高有关。糖尿病组和肥胖组的 hs-CRP 水平均较高，并且糖尿病组的 E-选择素水平显着较高。糖尿病组中 HbA1c 水平与 NTBI [P < 0.01]、HbA1c 与 E-选择素 [P < 0.001] 以及 NTBI 与 E-选择素 [P < 0.02] 呈强正相关。结论：这些结果支持铁介导的氧化应激可能是 2 型糖尿病血糖控制不良与血管功能障碍之间存在联系的机制这一假设。 (C) 2015 Elsevier Inc. 保留所有权利。
Aims: To investigate the association between circulating non-transferrin-bound iron [NTBI], and markers of oxidative stress, endothelial function and inflammation in subjects with type 2 diabetes and non-diabetic subjects with varying degrees of obesity.Methods: Plasma NTBI was measured by HPLC, together with total iron, iron-binding capacity, transferrin saturation and soluble transferrin receptor, together with total and reduced ascorbate, malondialdehyde [MDA], E-selectin and high-sensitivity c-reactive protein [hs-CRP] in groups of 28 subjects with type 2 diabetes, 28 non-obese controls and 17 obese non-diabetic subjects.Results: Levels of NTBI were higher than controls in the diabetes group, but the total serum iron levels were lower. MDA levels were higher than controls in both the diabetes and obese groups, and this was associated with higher levels of oxidised ascorbate. hs-CRP levels were higher in both the diabetes and obese groups, and E-selectin was significantly higher in the diabetes group. There were strong positive correlations between HbA1c levels and NTBI [P < 0.01], HbA1c and E-selectin [P < 0.001] and NTBI and E-selectin [P < 0.02] in the diabetes group.Conclusion: These results support the hypothesis that iron-mediated oxidative stress may be a mechanism linking poor glycaemic control with vascular dysfunction in type 2 diabetes. (C) 2015 Elsevier Inc. All rights reserved.