Endogenous and silencing-associated small RNAs in plants
Endogenous and silencing-associated small RNAs in plants
复制标题
DOI：
10.1105/tpc.003210
复制
发表时间：
2002-07-01
期刊：
影响因子：
11.6
通讯作者：
Carrington, JC
中科院分区：
文献类型：
--
作者：
Llave, C;Kasschau, KD;Carrington, JC
在拟南芥中鉴定出了一大类主要为21到24个核苷酸的内源性小RNA。这些小RNA与动物的微小RNA相似，并且在大小上与小干扰RNA相近；它们在由多种诱导物触发的RNA沉默过程中积累。在已鉴定的125个序列中，绝大多数（90%）来自基因间区域，尽管也鉴定出了与预测的蛋白质编码基因、类转座子序列以及一个结构RNA基因相对应的小RNA。通过对小RNA位点簇的鉴定和分析，获得了与两种极性的小RNA源自高度碱基配对的前体这一情况相符的证据。特定小RNA的积累受到发育调控。我们提出拟南芥小RNA参与广泛的转录后和表观遗传事件。
A large set of endogenous small RNAs of predominantly 21 to 24 nucleotides was identified in Arabidopsis. These small RNAs resembled micro-RNAs from animals and were similar in size to small interfering RNAs; that accumulated during RNA silencing triggered by multiple types of inducers. Among the 125 sequences identified, the vast majority (90%) arose from intergenic regions, although small RNAs corresponding to predicted protein-coding genes, transposon-like sequences, and a structural RNA gene also were identified. Evidence consistent with the derivation of small RNAs of both polarities, and from highly base-paired precursors, was obtained through the identification and analysis of clusters of small RNA loci. The accumulation of specific small RNAs was regulated developmentally. We propose that Arabidopsis small RNAs; participate in a wide range of post-transcriptional and epigenetic events.