An activity-based fluorescent probe and its application for differentiating alkaline phosphatase activity in different cell lines
An activity-based fluorescent probe and its application for differentiating alkaline phosphatase activity in different cell lines
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基于活性的荧光探针及其在区分不同细胞系碱性磷酸酶活性中的应用
DOI：
10.1039/d0cc06129h
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发表时间：
2020-11-11
影响因子：
4.9
通讯作者：
Sun, Hongyan
中科院分区：
文献类型：
--
作者：
He, Yong;Yu, Junli;Sun, Hongyan
本文报道了一种新的荧光探针AE-Phos，它结合了聚集诱导发射（AIE）和激发态分子内质子转移（ESIPT）的优点，用于检测ALP活性。进一步详细的荧光实验表明，AE-Phos表现出优异的选择性和灵敏度，大的斯托克斯位移，以及对ALP的快速响应。此外，AE-Phos被用于定量成像不同细胞系的ALP活性。
Herein, a new fluorescent probe, AE-Phos, is reported for detecting the ALP activity with the combined advantages of aggregation-induced emission (AIE) and excited state intramolecular proton transfer (ESIPT). Further detailed fluorescence experiments demonstrated that AE-Phos exhibited excellent selectivity and sensitivity, a large Stokes shift, and a fast response towards ALP. Furthermore, AE-Phos was applied to imaging the ALP activity in different cell lines quantitatively.