Virologic and Immunologic Evidence of Multifocal Genital Herpes Simplex Virus 2 Infection
Virologic and Immunologic Evidence of Multifocal Genital Herpes Simplex Virus 2 Infection
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DOI：
10.1128/jvi.03285-13
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发表时间：
2014-05-01
影响因子：
5.4
通讯作者：
Corey, Lawrence
中科院分区：
文献类型：
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作者：
Johnston, Christine;Zhu, Jia;Corey, Lawrence
生殖器单纯疱疹病毒（HSV）再激活被认为是解剖学和时间定位，符合有限的神经节感染。短暂的亚临床散毒发作是HSV-2再激活的最常见形式，宿主清除机制导致快速遏制。脱落发作的解剖学分布尚未得到表征。为了精确定义解剖学激活的模式，我们将生殖道分为22个区域网格，并在28个免疫活性，HSV-2血清阳性的人中从每个区域获得20天的每日拭子。通过PCR检测HSV，并在24小时内对无症状HSV脱落部位进行活检。通过免疫荧光定量CD 4（+）和CD 8（+）T细胞，并通过细胞内细胞因子细胞计数法鉴定HSV特异性CD 4（+）T细胞。在11，603份生殖器拭子中的868份（7%）中检测到HSV，中位数为每人12个部位（范围，0至22）。双侧HSV检出率为67%（83/83），平均日检出病毒量与阳性部位数相关（P
Genital herpes simplex virus (HSV) reactivation is thought to be anatomically and temporally localized, coincident with limited ganglionic infection. Short, subclinical shedding episodes are the most common form of HSV-2 reactivation, with host clearance mechanisms leading to rapid containment. The anatomic distribution of shedding episodes has not been characterized. To precisely define patterns of anatomic reactivation, we divided the genital tract into a 22-region grid and obtained daily swabs for 20 days from each region in 28 immunocompetent, HSV-2-seropositive persons. HSV was detected via PCR, and sites of asymptomatic HSV shedding were subjected to a biopsy procedure within 24 h. CD4(+) and CD8(+) T cells were quantified by immunofluorescence, and HSV-specific CD4(+) T cells were identified by intracellular cytokine cytometry. HSV was detected in 868 (7%) of 11,603 genital swabs at a median of 12 sites per person (range, 0 to 22). Bilateral HSV detection occurred on 83 (67%) days with shedding, and the median quantity of virus detected/day was associated with the number of sites positive (P