Amplification by long RT-PCR of near full-length norovirus genomes
Amplification by long RT-PCR of near full-length norovirus genomes
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DOI：
10.1016/j.jviromet.2008.02.001
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发表时间：
2008-05-01
影响因子：
3.1
通讯作者：
Tellier, Raymond
中科院分区：
文献类型：
--
作者：
Kostela, Jennifer;Ayers, Melissa;Tellier, Raymond
建立了长链RT-PCR扩增诺如病毒基因组的方法。从直接从临床样品中提取的RNA开始，并使用广泛反应性引物，它可以生成接近全长的扩增子，从而可以轻松确定接近完整的基因组序列。对2002年和2005年从加拿大多伦多分离的两株诺如病毒进行了测序。这种方法将有助于诺如病毒的分子流行病学研究。(c)2008 Elsevier B. V.保留所有权利。
A long RT-PCR method was developed to amplify the norovirus genome. Starting from RNA extracted directly from clinical samples and using broadly reactive primers, it can generate near full-length amplicons that allow for easy determination of the near complete genomic sequence. Two norovirus isolates from Toronto, Canada, in 2002 and 2005 were sequenced. This approach will facilitate molecular epidemiology studies of noroviruses. (c) 2008 Elsevier B.V. All rights reserved.