Primer-mediated enzymatic amplification of cytomegalovirus (CMV) DNA. Application to the early diagnosis of CMV infection in marrow transplant recipients.
Primer-mediated enzymatic amplification of cytomegalovirus (CMV) DNA. Application to the early diagnosis of CMV infection in marrow transplant recipients.
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巨细胞病毒 (CMV) DNA 的引物介导的酶扩增。
DOI：
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发表时间：
1989
影响因子：
15.9
通讯作者：
T. Pon
中科院分区：
文献类型：
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作者：
S. Cassol;Man;Poon;Margaret;J.;Naylor;J. Culver;Thomas;Bowen;James;A. Russell;Stephen;A. Krawetz;Richard;T. Pon
一种核酸扩增程序，聚合酶链反应（PCR），已被用来建立一个诊断检测巨细胞病毒（CMV）的临床标本中的立即早期序列的识别。对病毒感染的细胞培养物的初步测试表明，PCR检测是高度CMV特异性的，识别CMV的野生型和实验室菌株。与人类DNA或其他疱疹病毒的DNA没有交叉反应性。使用克隆的CMV AD 169 Eco RI片段-J作为模板，该测定的灵敏度为每40，000个细胞1个病毒基因组。在一项骨髓移植受者CMV感染的前瞻性研究中，PCR检测正确识别了4例确诊CMV感染的患者。在这些患者中的三个谁是纵向跟踪，DNA反应性与CMV培养和CMV抗体状态随时间的相关性表明，DNA是最敏感的标志物CMV感染的诊断。
A nucleic acid amplification procedure, the polymerase chain reaction (PCR), has been used to establish a diagnostic assay for the identification of cytomegalovirus (CMV) immediate-early sequences in clinical specimens. Preliminary testing against virus-infected cell cultures indicated that the PCR assay was highly CMV-specific, recognizing both wild-type and laboratory strains of CMV. There was no cross-reactivity with human DNA or with DNA from other herpes viruses. The sensitivity of the assay, using cloned CMV AD169 Eco RI fragment-J as template, was 1 viral genome per 40,000 cells. In a prospective study of CMV infection in bone marrow transplant recipients, the PCR assay correctly identified four patients with confirmed CMV infection. In three of these patients who were followed longitudinally, correlation of DNA reactivity with CMV culture and CMV antibody status over time indicated that DNA was the most sensitive marker for the diagnosis of CMV infection.