Temporal changes in synovial fluid composition and elastoviscous lubrication in the equine carpal fracture model
Temporal changes in synovial fluid composition and elastoviscous lubrication in the equine carpal fracture model
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DOI：
10.1002/jor.24281
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发表时间：
2019-05-01
影响因子：
2.8
通讯作者：
Reesink, Heidi L.
中科院分区：
文献类型：
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作者：
Feeney, Elizabeth;Peal, Bridgette T.;Reesink, Heidi L.
本研究的目的是检查关节骨折后滑液成分和润滑的时间变化。通过在四匹马的腕关节中部制造骨软骨骨折来诱导创伤后骨关节炎（PTOA），而对侧肢体作为假手术对照。马在高速跑步机上运动，并在术前和术后75天的连续时间点收集滑液。采用夹心elisa法测定润滑油素和透明质酸（HA）的浓度，凝胶电泳法分析HA的分子量分布。使用商用流变仪和定制的摩擦计分别在第0、19、33和61天测量所有润滑模式下的滑液粘度和软骨摩擦系数。术后血凝素浓度显著降低，高分子量血凝素（>6.1MDa）在第75天研究结束时未恢复到术前水平。与假手术肢体相比，OA手术后润滑素浓度增加的程度更大。术后黏度明显降低。虽然边界模态和弹粘模态摩擦系数没有变化，但表示这些模态之间转换的过渡数较低。虽然在OA肢体中更为明显，但假手术肢体中也观察到类似的HA、HA分子量分布、粘度和过渡数的紊乱，这可能与关节镜检查时滑液冲洗有关。(c) 2019年骨科研究学会。Wiley期刊公司出版。J骨科
The objective of this study was to examine temporal variations in synovial fluid composition and lubrication following articular fracture. Post-traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral limb served as a sham-operated control. Horses were exercised on a high-speed treadmill, and synovial fluid was collected pre-operatively and at serial timepoints until 75 days post-operatively. Lubricin and hyaluronic acid (HA) concentrations were measured using sandwich ELISAs, and the molecular weight distribution of HA was analyzed via gel electrophoresis. Synovial fluid viscosity and cartilage friction coefficients across all modes of lubrication were measured on days 0, 19, 33, and 61 using a commercial rheometer and a custom tribometer, respectively. HA concentrations were significantly decreased post-operatively, and high molecular weight HA (>6.1MDa) did not recover to pre-operative values by the study termination at day 75. Lubricin concentrations increased after surgery to a greater extent in the OA as compared to sham-operated limbs. Viscosity was significantly reduced after surgery. While boundary and elastoviscous mode friction coefficients did not vary, the transition number, representing the shift between these modes, was lower. Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy. (c) 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res