One-step microwave-assisted preparation of oxygen-rich multifunctional carbon quantum dots and their application for Cu2+-curcumin detection
One-step microwave-assisted preparation of oxygen-rich multifunctional carbon quantum dots and their application for Cu2+-curcumin detection
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微波辅助一步法制备富氧多功能碳量子点及其在Cu2-姜黄素检测中的应用
DOI：
10.1016/j.talanta.2019.120117
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发表时间：
2019
期刊：
影响因子：
6.1
通讯作者：
Huang Jing
中科院分区：
文献类型：
--
作者：
Luo Tao;Bu Lingli;Peng Siyu;Zhang Yaya;Zhou Zhi;Li Guorui;Huang Jing
碳量子点（CQDs）由于其独特的光学性质，在检测金属离子、有机小分子、大分子等方面取得了巨大的进展。CQD被证明是一种多功能探针，可用于通过不同的机制检测包括铜离子、生物硫醇和姜黄素在内的各种物质。通过荧光猝灭实现了对铜离子的选择性检测，其机理为静电猝灭。左旋CQDs-Cu ~（2+）体系的荧光可被生物硫醇（GSH、Cys和Hcy）恢复，表明左旋CQDs-Cu ~（2+）体系也可用于生物硫醇的检测。激发光谱与姜黄素的吸收峰有很好的重叠，通过内滤效应（IFE）荧光猝灭，可作为姜黄素的荧光探针。此外，左旋CQDs还可以通过还原左旋CQDs和Cu 2 +-姜黄素体系中CQDs的荧光来跟踪Cu 2 +-姜黄素复合物的形成，这一特性可用于Cu 2 +-姜黄素治疗阿尔茨海默病的机制研究。最后将该荧光左旋CQDs应用于不同类型的环境水样和人血清样品等真实的样品中Cu 2+和姜黄素的检测，为环境和生物样品中Cu 2+和姜黄素的检测提供了一种简便的方法。
Carbon quantum dots (CQDs), owing to its unique optical properties, have achieved tremendous progress for the detection of different metal ions, organic small molecules, macromolecules, etc. Here we synthesized fluorescent CQDs by a simple one-step microwave-assisted method using 3,4-Dihydroxy-l-phenylalanine (levodopa) as the carbon resource. The CQDs proved to be a multifunctional probe which can be used for detection of various species including copper ion, biothiols and curcumin, via different mechanisms. The selective detection of copper ion was obtained by fluorescence quenching and the mechanism was proved to be static quenching by electrostatic interaction. The fluorescence of levoCQDs-Cu2+system can be recovered by biothiols (GSH, Cys and Hcy), implying levoCQDs-Cu2+system can also be applied for biothiols detection. The excitation spectrum of levoCQDs had a good overlap with the absorption peak of curcumin, making it as a suitable curcumin probe by fluorescence quenching via inner filter effect (IFE). Furthermore, the levoCQDs can also track the formation of Cu2+-Curcumin complexes by restoring the fluorescence of the CQDs in levoCQDs and Cu2+-Curcumin system, and this feature may be exploited in the mechanism study of Cu2+-Curcumin in the treatment of Alzheimer's disease. The fluorescent levoCQDs were finally used for Cu2+and curcumin detection in some real samples including different types of environmental water samples and human serum samples, providing a convenient strategy to monitor Cu2+and curcumin in environmental and biological samples.