Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners
Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners
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DOI：
10.1186/s12970-020-00376-3
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发表时间：
2020-09-07
影响因子：
5.1
通讯作者：
Madsen, Klavs
中科院分区：
文献类型：
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作者：
Hansen, Mette;Oxfeldt, Mikkel;Madsen, Klavs
背景：蛋白质补充已被建议通过增加混合肌肉和肌纤维蛋白合成和瘦体重来增强耐力训练适应。然而，对线粒体适应性的潜在有益影响尚不清楚。本研究的目的是研究在为期六周的训练期间，在每次训练之后，与在相同时间摄入等热量的CHO补充剂相比，在线粒体生物发生、VO（2max）和训练跑步者的表现方面，在每次训练之前和之后摄入乳清蛋白水解物和乳清蛋白水解物加碳水化合物（PRO-CHO）的影响。方法：24名训练后的跑步者(VO(2max)60.7 +/- 3.7 ml O(2)kg（- 1) min(1)）完成为期6周的区域随机对照干预期，包括渐进式跑步训练。受试者被随机分配到PRO-CHO组或CHO组，并根据性别、年龄、最大摄氧量、训练和表现状况进行配对。PRO-CHO组在每次运动前摄入蛋白质饮料（0.3 g kg(- 1)），在每次运动后摄入蛋白质-碳水化合物饮料（0.3 g蛋白质kg（- 1）和1 g碳水化合物kg(- 1)）。CHO组摄入了能量匹配的碳水化合物饮料。对干预前后的静息肌肉活检进行线粒体特异性酶活性和线粒体蛋白含量分析。受试者在干预前、干预中途（仅干预6公里）和干预后分别完成6公里计时赛（6公里TT）和VO（2max）测试。结果：6周耐力训练后，PRO-CHO组细胞色素C （Cyt C）蛋白含量显著高于CHO组（p < 0.05），其他线粒体蛋白（琥珀酸脱氢酶（SDHA）、细胞色素C氧化酶（COX-IV）、电压依赖性阴离子通道（VDAC）、热休克蛋白60 （HSP60）和禁止蛋白（PHB1））也有类似的变化，但不显著（p = 0.07-0.14）。CHO组训练后β -羟酰基辅酶a脱氢酶（HAD）活性显著降低（p < 0.01），而PRO-CHO组训练后无显著降低（p = 0.24）。训练后VO（2max）和6k TT显著提高，组间无显著差异。结论：在为期六周的耐力训练期间，与摄入等热量的CHO相比，每次运动前摄入乳清PRO水解物以及每次运动后摄入乳清PRO水解物加CHO可能会增加对特定线粒体蛋白质的训练效果，但不会改变VO（2max）或6k TT的表现。
Background: Protein supplementation has been suggested to augment endurance training adaptations by increasing mixed muscle and myofibrillar protein synthesis and lean body mass. However, a potential beneficial effect on mitochondrial adaptations is yet to be clarified. The aim of the present study was to investigate the effect of consuming whey protein hydrolysate before and whey protein hydrolysate plus carbohydrate (PRO-CHO) after each exercise session during a six-week training period compared to similarly timed intake of isocaloric CHO supplements on biomarkers of mitochondrial biogenesis, VO(2max)and performance in trained runners.Methods: Twenty-four trained runners (VO(2max)60.7 +/- 3.7 ml O(2)kg(- 1) min(1)) completed a six-week block randomized controlled intervention period, consisting of progressive running training. Subjects were randomly assigned to either PRO-CHO or CHO and matched in pairs for gender, age, VO2max, training and performance status. The PRO-CHO group ingested a protein beverage (0.3 g kg(- 1)) before and protein-carbohydrate beverage (0.3 g protein kg(- 1)and 1 g carbohydrate kg(- 1)) after each exercise session. The CHO group ingested an energy matched carbohydrate beverage. Resting muscle biopsies obtained pre and post intervention were analyzed for mitochondrial specific enzyme activity and mitochondrial protein content. Subjects completed a 6 K time trial (6 K TT) and a VO(2max)test pre, midway (only 6 K TT) and post intervention.Results: Following six weeks of endurance training Cytochrome C (Cyt C) protein content was significantly higher in the PRO-CHO group compared to the CHO group (p < 0.05), with several other mitochondrial proteins (Succinate dehydrogenase (SDHA), Cytochrome C oxidase (COX-IV), Voltage-dependent anion channel (VDAC), Heat shock protein 60 (HSP60), and Prohibitin (PHB1)) following a similar, but non-significant pattern (p = 0.07-0.14). beta-hydroxyacyl-CoA dehydrogenase (HAD) activity was significantly lower after training in the CHO group (p < 0.01), but not in the PRO-CHO group (p = 0.24). VO(2max)and 6 K TT was significantly improved after training with no significant difference between groups.Conclusion: Intake of whey PRO hydrolysate before and whey PRO hydrolysate plus CHO after each exercise session during a six-week endurance training period may augment training effects on specific mitochondrial proteins compared to intake of iso-caloric CHO but does not alter VO(2max)or 6 K TT performance.