Comprehensive Glycan Analysis of Sphingolipids in Human Serum/Plasma.
Comprehensive Glycan Analysis of Sphingolipids in Human Serum/Plasma.
复制标题
人血清/血浆中鞘脂的综合聚糖分析。
DOI：
10.1007/978-1-0716-2910-9_21
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发表时间：
2023
影响因子：
--
通讯作者：
J. Furukawa
中科院分区：
文献类型：
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作者：
H. Hanamatsu;Takashi Nishikaze;J. Furukawa
鞘糖脂（GSL）是一种具有神经酰胺和碳水化合物头部基团的糖脂，在许多生物过程中发挥重要作用。以前，我们使用糖印迹法对各种细胞系和病毒感染的细胞进行了GSL-聚糖分析。最近，我们开发了几种唾液酸键特异性化学修饰的方法，以通过质谱法区分唾液酸化聚糖异构体。在本章中，我们描述了一种使用糖印迹结合氨解-SALSA（唾液酸键特异性烷基酰胺化）和内酯驱动的酯-酰胺衍生化（LEAD）-SALSA分析人血清/血浆中GSL-聚糖的方法，以获得全面和详细的结构糖组学。
Glycosphingolipids (GSLs) are glycolipids with ceramide and carbohydrate head groups that play an important role in numerous biological processes. Previously, we performed GSL-glycan analysis of various cell lines and virus-infected cells using a glycoblotting approach. Recently, we developed several methods for sialic acid linkage-specific chemical modification to distinguish sialylated glycan isomers by mass spectrometry. In this chapter, we describe a method for analyzing GSL-glycans in human serum/plasma using glycoblotting combined with aminolysis-SALSA (sialic acid linkage-specific alkylamidation) and lactone-driven ester-to-amide derivatization (LEAD)-SALSA for comprehensive and detailed structural glycomics.