Synthetic peptides with selective affinity for apoptotic cells
Synthetic peptides with selective affinity for apoptotic cells
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DOI：
10.1039/b514748d
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发表时间：
2006-01-01
影响因子：
3.2
通讯作者：
Smith, BD
中科院分区：
文献类型：
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作者：
DiVittorio, KM;Johnson, JR;Smith, BD
阴离子磷脂酰丝氨酸(PS)出现在细胞质膜的外单层，是细胞凋亡早期/中期的普遍指标。检测细胞表面PS最常见的方法是使用蛋白Annexin V；然而，在某些应用中，需要替代试剂。最近的研究表明，合理设计的2，2‘-二吡啶甲胺锌(Zn2+-DPA)配合物可以模拟膜联蛋白V的细胞凋亡传感功能。制备了上述标记的侧链含锌-DPA的三肽和五肽，并证明它们能选择性地结合到阴离子囊泡膜上。荧光素标记的多肽被用来通过荧光显微镜和流式细胞术检测凋亡细胞。
The appearance of anionic phosphatidylserine ( PS) in the outer monolayer of the plasma membrane is a universal indicator of the early/intermediate stages of cell apoptosis. The most common method of detecting PS on a cell surface is to use the protein annexin V; however, in certain applications there is a need for alternative reagents. Recent research indicates that rationally designed zinc 2,2'-dipicolylamine (Zn2+ - DPA) coordination complexes can mimic the apoptosis sensing function of annexin V. Here, a series of. uorescently-labelled, tri- and pentapeptides with side chains containing Zn2+ - DPA are prepared and shown to selectively bind to anionic vesicle membranes. Fluorescein-labelled versions of the peptides are used to detect apoptotic cells by fluorescence microscopy and flow cytometry.