Postmortem interval determination using 18S-rRNA and microRNA
Postmortem interval determination using 18S-rRNA and microRNA
复制标题
使用 18S-rRNA 和 microRNA 确定死后间隔。
DOI：
10.1016/j.scijus.2014.03.001
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发表时间：
2014-07-01
影响因子：
1.9
通讯作者：
Chen Long
中科院分区：
文献类型：
--
作者：
Li Wen-Can;Ma Kai-Jun;Chen Long
死亡时间的确定在刑事、民事和法医学案件中具有重要意义。PMI的精确估计是许多死亡调查的关键步骤。研究了利用RNA、18 S rRNA和microRNA水平来估计PMI的技术。18 S-rRNA是一种主要的核糖体RNA，作为核糖体蛋白复合物的一部分呈现，而microRNA是一类小的非编码单链RNA，只有21-25个核苷酸，在不同物种之间具有很强的保守性。在这项研究中，心脏组织从成年大鼠在不同的死后间隔。建立了一种高效的提取和检测方法，用于分析死后组织中18 S-rRNA和microRNA的水平。该过程包括总RNA提取、转录和通过定量真实的时间PCR的可视化。结果表明，死亡时间与死亡大鼠心脏18 S-rRNA的Ct值呈抛物线关系。结果表明，组织18 S-rRNA和microRNA的降解模式可用于推断死后7 d内的死亡时间。(C)2014年，特许法医学学会。由Elsevier爱尔兰有限公司出版。保留所有权利。
The importance of determining postmortem interval (PMI) is crucial to criminal, civil and forensic cases. The precise estimation of PMI is a critical step in many death investigations. A technique exploiting the level of RNA, 18S rRNA and microRNA to estimate PMI was investigated. 18S-rRNA is a main ribosomal RNA presented as part of the ribosomal protein complex, while microRNA is a class of small non-coding single-stranded RNA, only 21-25 nucleotides, which has a strong conservation between different species. In this study, heart tissues were removed from adult rats at various postmortem intervals. An efficient extraction and detection protocol to analyze the level of 18S-rRNA and microRNA in postmortem tissue was carried out. The process consists of total RNA extraction, transcription and visualization by quantitative real time PCR. The result indicates a characteristic parabola relationship between postmortem period and Ct values for 18S-rRNA in dead rat hearts. The result indicates that the degradation pattern of tissue 18S-rRNA and microRNA is useful in the determination of the postmortem interval within seven days. (C) 2014 The Chartered Society of Forensic Sciences. Published by Elsevier Ireland Ltd. All rights reserved.