Prediction of local structural stabilities of proteins from their amino acid sequences
Prediction of local structural stabilities of proteins from their amino acid sequences
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DOI：
10.1016/j.str.2006.12.007
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发表时间：
2007-02-01
期刊：
影响因子：
5.7
通讯作者：
Vendruscolo, Michele
中科院分区：
文献类型：
--
作者：
Tartaglia, Gian Gaetano;Cavalli, Andrea;Vendruscolo, Michele
氢交换实验提供了折叠蛋白、蛋白复合物和淀粉样原纤维结构不同区域的局部稳定性和溶剂可及性的详细信息。我们介绍了一种方法来预测保护因子从氢交换的蛋白质基于其氨基酸序列的知识，而不包括任何额外的结构信息。这些结果表明，球状蛋白结构的不同区域发生局部展开事件的倾向性可以从它们的氨基酸序列中预测，准确率为80%或更高。
Hydrogen exchange experiments provide detailed information about the local stability and the solvent accessibility of different regions of the structures of folded proteins, protein complexes, and amyloid fibrils. We introduce an approach to predict protection factors from hydrogen exchange in proteins based on the knowledge of their amino acid sequences without the inclusion of any additional structural information. These results suggest that the propensity of different regions of the structures of globular proteins to undergo local unfolding events can be predicted from their amino acid sequences with an accuracy of 80% or better.