Insulin metabolism in human adipocytes from subcutaneous and visceral depots
Insulin metabolism in human adipocytes from subcutaneous and visceral depots
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DOI：
10.1016/j.bbrc.2010.10.104
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发表时间：
2010-11-26
影响因子：
3.1
通讯作者：
Duckworth, William C.
中科院分区：
文献类型：
--
作者：
Fawcett, Janet;Sang, Hairong;Duckworth, William C.
代谢综合征(胰岛素抵抗、糖耐量异常、高血压等)患者腹部脂肪组织相对增多，肥胖也与胰岛素清除减少有关。胰岛素清除的大部分是由于胰岛素降解酶(IDE)的作用，且所有组织中都存在IDE。由于肥胖患者腹部脂肪增加，我们假设IDE可能在不同的脂肪库中发生改变。从择期腹部手术受试者的脂肪样本中分离脂肪细胞。脂肪样本取自皮下(SQ)和内脏(VIS)部位。从SQ和VIS脂肪组织分离的脂肪细胞比从SQ脂肪组织分离的脂肪细胞降解更多的胰岛素。组织蛋白酶B和D的抑制剂对胰岛素的降解没有影响，而杆菌肽(一种IDE的抑制剂)抑制了SQ和VIS脂肪细胞的降解约33%。这些数据表明，VIS的胰岛素代谢相对比SQ脂肪组织强，可能是由于Elsevier公司发表的IDE所致
Subjects with the metabolic syndrome (insulin resistance glucose intolerance dyslipidemia hypertension etc) have a relative increase in abdominal fat tissue compared to normal individuals and obesity has also been shown to be associated with a decrease in insulin clearance The majority of the clearance of insulin is due to the action of insulin-degrading enzyme (IDE) and IDE is present throughout all tissues Since abdominal fat is increased in obesity we hypothesized that IDE may be altered in the different fat depots Adipocytes were isolated from fat samples obtained from subjects during elective abdominal surgery Fat samples were taken from subcutaneous (SQ) and visceral (VIS) sites Insulin metabolism was compared in adipocytes isolated from SQ and VIS fat tissue Adipocytes from the VIS site degraded more insulin that those from SQ fat tissue Inhibitors of cathepsins B and D has no effect on the degradation of insulin while bacitracin an inhibitor of IDE inhibited degradation by approx 33% in both SQ and VIS adipocytes These data show that insulin metabolism is relatively greater in VIS than in SQ fat tissue and potentially due to IDE Published by Elsevier Inc