The genome and proteome of Serratia bacteriophage η which forms unstable lysogens.
The genome and proteome of Serratia bacteriophage η which forms unstable lysogens.
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DOI：
10.1186/1743-422x-11-6
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发表时间：
2014-01-16
期刊：
影响因子：
4.8
通讯作者：
Kropinski AM
中科院分区：
文献类型：
--
作者：
Denyes JM;Krell PJ;Manderville RA;Ackermann HW;She YM;Kropinski AM
粘质沙雷氏菌噬菌体η是一种温和的未分类的Siphoviridae成员，已报道含有高修饰的鸟嘌呤残基。通过酶切、高效液相色谱分析核苷组成、DNA测序和蛋白质组学分析对DNA进行了鉴定。还研究了其形成稳定溶原和整合的能力。酶切和HPLC分析表明，噬菌体η DNA不含修饰碱基。该病毒的基因组序列经焦磷酸测序测定，长度为42,724个核苷酸，摩尔% GC为49.9，呈循环排列。鉴定了69个假定的cds，其中19个编码新的蛋白质。虽然鉴定出7个近亲，但它们仅与噬菌体η基因组的基因40 ~ 69具有序列相似性，而gp1 ~ gp39没有保守关系。结构蛋白质组通过SDS-PAGE和质谱分析发现了7种独特的蛋白质。这种噬菌体与宿主粘质葡萄球菌形成非常不稳定的溶菌原。
Serratia marcescens phage η is a temperate unclassified member of the Siphoviridae which had been reported as containing hypermodified guanine residues. The DNA was characterized by enzymatic digestion followed by HPLC analysis of the nucleoside composition, and by DNA sequencing and proteomic analysis. Its ability to form stable lysogens and integrate was also investigated. Enzymatic digestion and HPLC analysis revealed phage η DNA did not contain modified bases. The genome sequence of this virus, determined using pyrosequencing, is 42,724 nucleotides in length with a mol% GC of 49.9 and is circularly permuted. Sixty-nine putative CDSs were identified of which 19 encode novel proteins. While seven close genetic relatives were identified, they shared sequence similarity with only genes 40 to 69 of the phage η genome, while gp1 to gp39 shared no conserved relationship. The structural proteome, determined by SDS-PAGE and mass spectrometry, revealed seven unique proteins. This phage forms very unstable lysogens with its host S. marcescens.