Mussel-Inspired Polydopamine: A Biocompatible and Ultrastable Coating for Nanoparticles in Vivo
Mussel-Inspired Polydopamine: A Biocompatible and Ultrastable Coating for Nanoparticles in Vivo
复制标题
受贻贝启发的聚多巴胺：一种生物相容且超稳定的体内纳米颗粒涂层
DOI：
10.1021/nn404117j
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发表时间：
2013-10-01
期刊：
影响因子：
17.1
通讯作者：
Ji, Jian
中科院分区：
文献类型：
--
作者：
Liu, Xiangsheng;Cao, Jieming;Ji, Jian
生物启发聚多巴胺（PDA）已作为一种通用的涂层纳米粒子（NP）的各种生物医学应用。然而，一个剩下的关键问题是纳米颗粒上的PDA壳在体内是否稳定。在这项研究中，我们修改了金纳米粒子（GNP）与精细控制的PDA纳米层，形成均匀的核/壳纳米结构（GNP@PDA）。体外研究表明，PDA包被的GNP具有低细胞毒性，并能顺利地转移到癌细胞中。透射电子显微镜（TEM）分析表明，PDA纳米壳在24小时孵育后在细胞内是完整的。值得注意的是，我们发现GNP@PDA可以部分地从内体/溶酶体逃逸到胞质溶胶并定位在细胞核附近。此外，我们观察到PDA包被的NP在肝脏和脾脏的两个重要器官中具有非常不同的摄取行为：肝脏中的GNP@PDA主要被枯否细胞摄取，而脾脏中的GNP@PDA被多种细胞摄取。重要的是，我们证明了PDA纳米壳在肝脏和脾脏的细胞内稳定至少六周，并且GNP@PDA在长时间内对小鼠的主要器官没有显示出显著的组织学毒性。这些结果提供了直接证据支持PDA表面修饰可以作为一种有效的策略，在体内形成超稳定的涂层，这可以提高细胞内递送能力和生物相容性的纳米粒子的生物医学应用。
Bioinspired polydopamine (PDA) has served as a universal coating to nanoparticles (NPs) for various biomedical applications. However, one remaining critical question is whether the PDA shell on NPs is stable in vivo. In this study, we modified gold nanoparticles (GNPs) with finely controlled PDA nanolayers to form uniform core/shell nanostructures (GNP@PDA). In vitro study showed that the PDA-coated GNPs had low cytotoxicity and could smoothly translocate to cancer cells. Transmission electron microscopy (TEM) analysis demonstrated that the PDA nanoshells were intact within cells after 24 h incubation. Notably, we found the GNP@PDA could partially escape from the endosomes/lysosomes to cytosol and locate close to the nucleus. Furthermore, we observed that the PDA-coated NPs have very different uptake behavior in two Important organs of the liver and spleen: GNP@PDA in the liver were mainly uptaken by the Kupffer cells, while the GNP@PDA in the spleen were uptaken by a variety of cells. Importantly, we proved the PDA nanoshells were stable within cells of the liver and spleen for at least six weeks, and GNP@PDA did not show notable histological toxicity to main organs of mice in a long time. These results provided the direct evidence to support that the PDA surface modification can serve as an effective strategy to form ultrastable coatings on NPs in vivo, which can improve the intracellular delivery capacity and biocompatibility of NPs for biomedical application.