E-selectin appears in nonischemic tissue during experimental focal cerebral ischemia
E-selectin appears in nonischemic tissue during experimental focal cerebral ischemia
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DOI：
10.1161/01.str.27.8.1386
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发表时间：
1996-08-01
期刊：
影响因子：
8.3
通讯作者：
delZoppo, GJ
中科院分区：
文献类型：
--
作者：
Haring, HP;Berg, EL;delZoppo, GJ
背景与目的E-选择素参与了局灶性脑缺血再灌注后白细胞-内皮细胞的黏附和炎症过程。方法在非人类灵长类动物模型上，观察大脑中动脉闭塞2小时(n=4)和缺血再灌流3 h(1 h，n=4；4 h，n=6；24 h，n=6)内E-选择素抗原的表达和去向。结果与可溶性重组人E-选择素孵育后，E-1E4抗体所定义的微血管E-选择素表达有三种模式：(1)缺血后2小时至再灌流4小时，E-选择素/层粘连蛋白呈低水平表达(E-选择素/层粘连蛋白=0.32+/-0.10)。(2)与对照组和非缺血组织相比，再灌流24小时的缺血微血管中有相当一部分显示高强度的E-选择素信号(0.61+/-0.17)(P
Background and Purpose E-selectin participates in leukocyte-endothelial adhesion and the inflammatory processes that follow focal cerebral ischemia and reperfusion. The temporal and topographical patterns of microvascular E-selectin presentation after experimental focal cerebral ischemia are relevant to microvascular reactivity to ischemia.Methods The upregulation and fate of E-selectin antigen during 2 hours of middle cerebral artery occlusion (n=4) and 3 hours of occlusion with reperfusion (1 hour, n=4; 4 hours, n=6; 24 hours, n=6) were evaluated in the nonhuman primate. E-selectin and E:P-selectin immunoreactivities were semiquantitated with the use of computerized light microscopy video imaging and laser confocal microscopy.Results Three patterns of microvascular E-selectin expression, defined by the antibody E-1E4, were confirmed by complete elimination of E-1E4 binding after incubation with soluble recombinant human E-selectin: (1) Low immunoperoxidase intensity was observed in ischemic microvessels at 2 hours of occlusion extending to 4 hours of reperfusion (E-selectin/laminin = 0.32+/-0.10). (2) A significant fraction of ischemic microvessels displayed high-intensity E-selectin signal by 24 hours of reperfusion (0.61+/-0.17) compared with control and nonischemic tissues (2P