LmCDA1 organizes the cuticle by chitin deacetylation in Locusta migratoria
LmCDA1 organizes the cuticle by chitin deacetylation in Locusta migratoria
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DOI：
10.1111/imb.12554
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Jianzhen Zhang
中科院分区：
文献类型：
--
作者：
Rongrong Yu;Weimin Liu;Xiaoming Zhao;Min Zhang;DaQi Li;Renata Zuber;Enbo Ma;Kun Yan Zhu;Bernard Moussian;Jianzhen Zhang
Cells produce an extracellular matrix (ECM) with a stereotypic organization that is important for tissue function. The insect cuticle is a layered ECM that mainly consists of the polysaccharide chitin and associated proteins adopting a quasi-crystalline structure. Our understanding of the molecular mechanisms deployed during construction of the highly ordered protein–chitin ECM so far is limited. In this study, we report on the role of the chitin deacetylase 1 (LmCDA1) in the organization of the protein–chitin ECM in the migratory locust Locusta migratoria, and LmCDA1 localizes predominantly to the apical tier of the protein–chitin ECM, but it is also found in lower regions. Reduction of LmCDA1 function correlates with lower amounts of chitin and impedes conversion of chitin to chitosan by deacetylation. Establishment of the quasi-crystalline architecture of the protein–chitin ECM is, however, independent of LmCDA1 activity, but it is dependent on another chitin deacetylase, LmCDA2, which has no detectable effects on chitin deacetylation and, as shown previously, no influence on chitin content. Our data reveal that LmCDA1 and LmCDA2 act in parallel and independently from each other in defining the dimensions of the cuticle. Both enzymes are non-uniformly distributed within the protein–chitin matrix, suggesting a site-autonomous function.
