Proteomic and phosphoproteomic determination of ABA's effects on grain-filling of Oryza sativa L. inferior spikelets.
Proteomic and phosphoproteomic determination of ABA's effects on grain-filling of Oryza sativa L. inferior spikelets.
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DOI：
10.1016/j.plantsci.2011.11.012
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发表时间：
2012-04
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通讯作者：
Zhi-xing Zhang;Jun Chen;Shisheng Lin;Zhong Li;Rong-Qiang Cheng;C. Fang;Hongfei Chen;Wenxiong Lin-Wenxion
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文献类型：
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作者：
Zhi-xing Zhang;Jun Chen;Shisheng Lin;Zhong Li;Rong-Qiang Cheng;C. Fang;Hongfei Chen;Wenxiong Lin-Wenxion
水稻品种（Oryza sativa L.），尤其是大小穗型，往往由于其劣质（晚花）小穗灌浆不良而无法实现高产潜力。上位（早花）小穗通常比下位小穗含有更多的脱落酸（ABA）。据推测，ABA 可能在劣质小穗的籽粒灌浆中发挥关键作用。为了了解这一过程中涉及的分子调控，我们采用基于二维凝胶的比较蛋白质组学和磷酸化蛋白质组学分析来寻找外源 ABA 处理下的下小穗中差异表达的蛋白质。处理后的下小穗中共发现111个显着差异蛋白和31个磷蛋白。其中，利用MALDI-TOF/TOF MS鉴定出100种蛋白质和23种磷蛋白。此外，通过 RT-PCR 证实了下小穗的基因表达模式。这些差异表达的蛋白质在防御反应、碳水化合物、蛋白质、氨基酸、能量和次级代谢以及细胞发育和光合作用中活跃。结果表明，水稻劣质小穗的灌浆过程是由ABA通过一些参与碳、氮和能量代谢的蛋白质和磷蛋白来调控的。
Cultivars of rice (Oryza sativa L.), especially the large-spikelet-type, often fail to achieve the high yield potential due to poor grain-filling of their inferior (late-flowering) spikelets. The superior (early-flowering) spikelets normally contain more abscisic acid (ABA) than the inferior spikelets. It was speculated that ABA might play a pivotal role in the grain-filling of inferior spikelets. To understand the molecular regulation involved in this process, we employed the 2-D gel-based comparative proteomic and phosphoproteomic analyses to search for differentially expressed proteins in the inferior spikelets under exogenous ABA treatment. A total of 111 significantly differential proteins and 31 phosphoproteins were found in the inferior spikelets after treatment. Among them, 100 proteins and 23 phosphoproteins were identified by using MALDI-TOF/TOF MS. In addition, the gene expression patterns of the inferior spikelets were confirmed with RT-PCR. These differentially expressed proteins are active in defense response, carbohydrate, protein, amino acid, energy and secondary metabolisms, as well as cell development and photosynthesis. The results suggest that the grain-filling of rice inferior spikelets is regulated by ABA through some proteins and phosphoproteins participating in carbon, nitrogen and energy metabolisms.