Novel systemic lupus erythematosus autoantigens identified by human protein microarray technology
Novel systemic lupus erythematosus autoantigens identified by human protein microarray technology
复制标题
通过人类蛋白质微阵列技术鉴定出新型系统性红斑狼疮自身抗原
DOI：
10.1016/j.bbrc.2012.01.001
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发表时间：
2012-02-10
影响因子：
3.1
通讯作者：
Wu, Lin
中科院分区：
文献类型：
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作者：
Huang, Wei;Hu, Chaojun;Wu, Lin
系统性红斑狼疮(SLE)是一种累及多器官的全身性自身免疫性疾病。许多自身抗体都与该病有关，但要么特异性低，要么检测灵敏度低。为了筛选更好的自身抗体，我们使用包含5011个非冗余人类蛋白的蛋白质芯片分析了30名SLE患者和30名健康对照血清中的自身抗体谱，并确定了四个候选。然后，我们选择CLIC2在扩大的队列中进行进一步的ELISA验证，该队列包括110名SLE患者、121名非AD患者、118名RA患者、117名SSC患者和105名PSS患者。SLE患者抗CLIC2抗体阳性率为28.18%，显著高于非AD、RA和SSC患者。SLE患者抗CLIC2抗体的存在与SLEDAI评分及多项指标呈正相关(p<；0.05)。(C)2012 Elsevier Inc.保留所有权利。
Systemic lupus erythematosus (SLE) is a systemic autoimmune disease affecting many organs. Many autoantibodies have been associated with the disease, but either in low specificity or low sensitivity of detection. In an aim to screen for better autoantibodies, we profiled the autoantibody repertoire in sera from 30 SLE patients versus 30 healthy controls using a protein microarray containing 5011 non-redundant human proteins, and identified four candidates. We then selected CLIC2 for further verification by ELISA in an extended cohort including 110 SLE, 121 non-AD, 118 RA, 117 SSc, and 105 pSS patients. The positive rate of anti-CLIC2 was 28.18% in SLE patients, significantly higher than those in non-AD, RA, and SSc patients. The presence of anti-CLIC2 in SLE had positive correlation with disease activity in terms of SLEDAI score and several indexes (p < 0.05). (C) 2012 Elsevier Inc. All rights reserved.