Ion transporter gene expression is linked to the thermal sensitivity of calcification in the reef coral Stylophora pistillata
Ion transporter gene expression is linked to the thermal sensitivity of calcification in the reef coral Stylophora pistillata
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DOI：
10.1038/s41598-019-54814-7
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发表时间：
2019-12-10
影响因子：
4.6
通讯作者：
Venn, A. A.
中科院分区：
文献类型：
--
作者：
Bernardet, C.;Tambutte, E.;Venn, A. A.
珊瑚钙化是生物多样性珊瑚礁生态系统的基础，但珊瑚对海水温度变化的热敏感性背后的生理学尚不清楚。此外，光也是调节钙化率的关键因素，但缺乏对光与温度相互作用影响珊瑚钙化的机制理解。本研究对分布广泛的模式珊瑚柱头珊瑚（Stylophora pisttillata）钙化的热性能曲线（TPC）进行了表征，并利用基因表达分析研究了离子传输机制在昼夜钙化热驱动下降中的作用。我们的研究重点是与溶解无机碳（DIC）、钙和H+运输相关的基因，揭示了生理反应（如钙化和呼吸）在白天和夜间条件下不同温度下具有不同基因表达模式的高度一致性。在低温下，与DIC运输过程相关的钙化和基因表达下调，但对光线的反应不大。相比之下，在高温下，光对钙化有积极的影响，并刺激了离子转运体功能更多样化的基因表达反应。总的来说，我们的研究结果强调了与DIC、钙和H+运输相关的机制在珊瑚钙化的热敏性中的作用，以及这种敏感性如何受到光的影响。
Coral calcification underpins biodiverse reef ecosystems, but the physiology underlying the thermal sensitivity of corals to changing seawater temperatures remains unclear. Furthermore, light is also a key factor in modulating calcification rates, but a mechanistic understanding of how light interacts with temperature to affect coral calcification is lacking. Here, we characterized the thermal performance curve (TPC) of calcification of the wide-spread, model coral species Stylophora pistillata, and used gene expression analysis to investigate the role of ion transport mechanisms in thermally-driven declines in day and nighttime calcification. Focusing on genes linked to transport of dissolved inorganic carbon (DIC), calcium and H+, our study reveals a high degree of coherence between physiological responses (e.g. calcification and respiration) with distinct gene expression patterns to the different temperatures in day and night conditions. At low temperatures, calcification and gene expression linked to DIC transport processes were downregulated, but showed little response to light. By contrast, at elevated temperature, light had a positive effect on calcification and stimulated a more functionally diverse gene expression response of ion transporters. Overall, our findings highlight the role of mechanisms linked to DIC, calcium and H+ transport in the thermal sensitivity of coral calcification and how this sensitivity is influenced by light.