Evolution of a novel subfamily of nuclear receptors with members that each contain two DNA binding domains.
Evolution of a novel subfamily of nuclear receptors with members that each contain two DNA binding domains.
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DOI：
10.1186/1471-2148-7-27
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发表时间：
2007-02-23
影响因子：
3.4
通讯作者：
LoVerde PT
中科院分区：
文献类型：
--
作者：
Wu W;Niles EG;Hirai H;LoVerde PT
核受体（Nuclear receptors，NRs）是后生动物重要的转录调节因子，通过DNA结合域（DNA binding domain，DBD）与靶基因启动子区结合，并通过与配体结合域（ligand binding domain，LBD）结合的辅助调节因子激活或抑制mRNA的合成来调节转录。NR通常具有带有LBD的单个DBD。从曼氏血吸虫（Schistosoma mansoni）中鉴定出三种核受体，命名为2DBD-NRs，每种受体具有一组新的串联的两个DBD和一个LBD。它们代表了一种新的NR模块化结构：A/B-DBD-DBD-铰链-LBD。2DBD-NR形成NR的新亚家族VII。通过数据库挖掘，还从其他扁虫（Schmidtea meditagleea和Dugesia japonica）、软体动物（Lottia acutetean）和节肢动物（Daphnia pulex）中鉴定了2个DBD-NR基因。所有2DBD-NR在第一个DBD中具有CEACKK的P盒序列，这是2DBD-NR所特有的，并且在第二个DBD中具有CEGCKG的P盒序列。DBD和配体结合域序列的系统发育分析表明，2DBD-NR基因起源于一个共同的两个DBD的祖先基因。在节肢动物、扁形动物和软体动物中发现了单一的2DBD-NR直向同源物。随后在软体动物和扁形动物中的2DBD-NR基因进化涉及基因复制。染色体定位。mansoni 2DBD-NR基因的荧光原位杂交（FISH）结果表明，2DBD-NR基因在扁形动物的不同染色体上复制。Sm 2DBD α的二聚化表明2DBD-NR可能作为同源二聚体，这表明2DBD-NR的每个DBD与其靶基因结合需要两个重复的半位点，或者每个2DBD-NR可以识别多个位点。2DBD-NR具有共同的祖先基因，其具有可能由重组事件导致的额外DBD。在节肢动物、软体动物和扁形动物分离后，2DBD-NR在软体动物的共同祖先中经历了最近的复制，而在扁形动物的共同祖先中发生了两轮复制。这表明，某些NR基因经历了最近的复制后，前列腺口和后口分裂的前列腺口谱系。
Nuclear receptors (NRs) are important transcriptional modulators in metazoans which regulate transcription through binding to the promoter region of their target gene by the DNA binding domain (DBD) and activation or repression of mRNA synthesis through co-regulators bound to the ligand binding domain (LBD). NRs typically have a single DBD with a LBD. Three nuclear receptors named 2DBD-NRs, were identified from the flatworm Schistosoma mansoni that each possess a novel set of two DBDs in tandem with a LBD. They represent a novel NR modular structure: A/B-DBD-DBD-hinge-LBD. The 2DBD-NRs form a new subfamily of NRs, VII. By database mining, 2DBD-NR genes from other flatworm species (Schmidtea mediterranea and Dugesia japonica), from Mollusks (Lottia gigantean) and from arthropods (Daphnia pulex) were also identified. All 2DBD-NRs possess a P-box sequence of CEACKK in the first DBD, which is unique to 2DBD-NRs, and a P-box sequence of CEGCKG in the second DBD. Phylogenetic analyses of both DBD and ligand binding domain sequences showed that 2DBD-NR genes originate from a common two DBD-containing ancestor gene. A single 2DBD-NR orthologue was found in Arthropoda, Platyhelminths and Mollusca. Subsequent 2DBD-NR gene evolution in Mollusks and Platyhelminths involved gene duplication. Chromosome localization of S. mansoni 2DBD-NR genes by Fluorescent in situ hybridization (FISH) suggests that 2DBD-NR genes duplicated on different chromosomes in the Platyhelminths. Dimerization of Sm2DBDα indicates that 2DBD-NRs may act as homodimers, suggesting either that two repeats of a half-site are necessary for each DBD of 2DBD-NRs to bind to its target gene, or that each 2DBD-NR can recognize multiple sites. 2DBD-NRs share a common ancestor gene which possessed an extra DBD that likely resulted from a recombination event. After the split of the Arthropods, Mollusks and Platyhelminths, 2DBD-NR underwent a recent duplication in a common ancestor of Mollusks, while two rounds of duplication occurred in a common ancestor of the Platyhelminths. This demonstrates that certain NR gene underwent recent duplication in Prostostome lineages after the split of the Prostostomia and Deuterostomia.
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DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
DOI：
10.1046/j.1432-1327.2000.01344.x
发表时间：
2000-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
de Mendonça, RL;Escriva, H;Laudet, V
通讯作者：
Laudet, V
影响因子：
16
作者：
Bourguet, W;Vivat, V;Moras, D
通讯作者：
Moras, D
影响因子：
2.5
作者：
Jondelius, U;Ruiz-Trillo, I;Riutort, M
通讯作者：
Riutort, M
影响因子：
4.8
作者：
Freebern, WJ;Osman, A;LoVerde, PT
通讯作者：
LoVerde, PT