What is the Most Sensitive Measure of Water Maze Probe Test Performance?
What is the Most Sensitive Measure of Water Maze Probe Test Performance?
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DOI：
10.3389/neuro.07.004.2009
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发表时间：
2009
影响因子：
3.5
通讯作者：
Frankland PW
中科院分区：
文献类型：
--
作者：
Maei HR;Zaslavsky K;Teixeira CM;Frankland PW
水迷宫通常用于测定实验啮齿动物模型中的空间认知，或者更一般地，学习和记忆。在水迷宫中，小鼠或大鼠被训练导航到位于水面以下的平台。然后通常在探测测试中评估空间学习，其中从池中移除平台并且允许小鼠或大鼠搜索它。（在以先前平台位置为中心的虚拟象限[Q]或区域[Z]中的时间百分比），基于误差（与先前平台位置的平均接近度[P]）或基于计数（平台交叉点[X]）的测量。虽然这些措施在他们的普及程度不同，他们是否在他们的能力不同检测组的差异是未知的。为了解决这个问题，我们编制了五个独立的数据库，包含超过1600个鼠标探针测试。从各自的数据库中随机选择单个试验，然后允许我们模拟不同样本和效应大小的实验。使用这种基于蒙特卡罗的方法，我们发现P测量在检测组差异的能力方面始终优于Q，Z和X测量。无论样本量或效应量如何，均采用参数和非参数统计分析。P比其他常用的措施的相对优势表明，它是最合适的措施，采用在低通量和高通量水迷宫屏幕。
The water maze is commonly used to assay spatial cognition, or, more generally, learning and memory in experimental rodent models. In the water maze, mice or rats are trained to navigate to a platform located below the water's surface. Spatial learning is then typically assessed in a probe test, where the platform is removed from the pool and the mouse or rat is allowed to search for it. Performance in the probe test may then be evaluated using either occupancy-based (percent time in a virtual quadrant [Q] or zone [Z] centered on former platform location), error-based (mean proximity to former platform location [P]) or counting-based (platform crossings [X]) measures. While these measures differ in their popularity, whether they differ in their ability to detect group differences is not known. To address this question we compiled five separate databases, containing more than 1600 mouse probe tests. Random selection of individual trials from respective databases then allowed us to simulate experiments with varying sample and effect sizes. Using this Monte Carlo-based method, we found that the P measure consistently outperformed the Q, Z and X measures in its ability to detect group differences. This was the case regardless of sample or effect size, and using both parametric and non-parametric statistical analyses. The relative superiority of P over other commonly used measures suggests that it is the most appropriate measure to employ in both low- and high-throughput water maze screens.