Inhibition of the processing of ribosomal precursor RNA by intercalating agents.
Inhibition of the processing of ribosomal precursor RNA by intercalating agents.
复制标题
嵌入剂抑制核糖体前体 RNA 的加工。
DOI：
10.1016/0022-2836(71)90371-8
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发表时间：
1971
影响因子：
5.6
通讯作者：
K. Kohn
中科院分区：
文献类型：
--
作者：
A. L. Snyder;H. Kann;K. Kohn
在L1210小鼠淋巴瘤细胞中，Proflavin、ethidium和ellipticine这三种已知或被认为通过嵌入与核酸螺旋结合的化合物被发现可以抑制核糖体RNA成熟序列中45 s核仁RNA向其他RNA物种的加工。这种作用是迅速的，作用于正常形成的45s核核RNA，而不是继发于RNA或蛋白质合成的抑制；因此，它似乎是由于直接作用于核仁RNA加工。这些发现提示在45s核仁RNA的特定加工位点可能存在螺旋碱基对片段。
Proflavin, ethidium, and ellipticine, compounds known or thought to bind to nucleic acid helices by intercalation, were found to inhibit the processing of 45 s nucleolar RNA to other RNA species in the ribosomal RNA maturation sequence in L1210 mouse lymphoma cells. The effect is prompt, is exerted on normally formed 45 s nucleolar RNA, and is not secondary to inhibition of RNA or protein synthesis; hence it appears to be due to direct action on nucleolar RNA processing. These findings suggest the possible existence of helical basepaired segments at specific processing sites in 45 s nucleolar RNA.