Osmotic stabilizer-coupled suppression of NDR defects is dependent on the calcium-calcineurin signaling cascade in Aspergillus nidulans
Osmotic stabilizer-coupled suppression of NDR defects is dependent on the calcium-calcineurin signaling cascade in Aspergillus nidulans
复制标题
NDR 缺陷的渗透稳定剂偶联抑制依赖于构巢曲霉中的钙-钙调神经磷酸酶信号级联
DOI：
10.1016/j.cellsig.2011.06.009
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发表时间：
2011-11-01
影响因子：
4.8
通讯作者：
Lu, Ling
中科院分区：
文献类型：
--
作者：
Gao, Lina;Song, Yiju;Lu, Ling
细胞极性的建立和维持是由信号通路如NDR（核Dbf 2相关）蛋白激酶信号通路和钙信号通路协调的。NDR激酶家族在结构上与人类强直性肌营养不良激酶相关，当其受损时，会导致涉及细胞结构和离子稳态变化的疾病。科塔激酶是NDR蛋白激酶家族的成员，与MobB形成复合物以调节构巢曲霉中细胞的极化生长。我们以前的研究表明，mobB/科塔缺陷可以抑制的渗透压在存在外部钙。在本研究中，通过遗传和分子方法，我们进一步证明了渗透稳定剂偶联抑制需要Ca 2+渗透性牵张激活非选择性阳离子通道-MidA、钙/钙调素依赖性蛋白磷酸酶催化亚基-CnaA和外部钙，而不是电压门控钙通道同源物-CchA。渗透胁迫诱导的钙/钙调神经磷酸酶信号通路的上调可能是绕过NDR激酶复合物的需要的原因，而NDR激酶复合物是极性形态发生所必需的。我们的研究结果表明，钙-钙调神经磷酸酶信号通路与MobB/科塔激酶复合物协调，通过维持细胞内钙稳态来调节极性生长。然而，CchA在构巢曲霉中可能与钙信号通路中的其他组分不同。这些发现提供了一个很好的机会，以确定潜在的途径连接NDR蛋白激酶网络的钙信号通路。(C)2011 Elsevier Inc. All rights reserved.
Establishment and maintenance of cell polarity are coordinated by signaling pathways such as NDR (nuclear Dbf2-related) protein-kinase signaling and calcium signaling pathway. The NDR family of kinase is structurally related to the human myotonic dystrophy kinase, which, when impaired, confers a disease that involves changes in cytoarchitecture and ion homeostasis. CotA kinase, a member of the NDR protein kinase family, forms a complex with MobB to regulate cell polarized growth in Aspergillus nidulans. Our previous study demonstrated that mobB/cotA defects could be suppressed by the osmotic stress in the presence of external calcium. In this study, via the genetic and molecular approach, we further demonstrated that Ca2+-permeable stretch-activated nonselective cation channel-MidA, calcium/calmodulin-dependent protein phosphatase catalatic subunit-CnaA and external calcium, but not voltage-gated calcium channel homolog-CchA, were required for the osmotic stabilizer-coupled suppression. The up-regulation of calcium/calcineurin signaling pathway induced by osmotic stress might be the reason for bypassing the requirements of NDR kinase complex, which is otherwise necessary for polar morphogenesis. Our results suggest that calcium-calcineurin signaling pathway coordinates with MobB/CotA kinase complex in regulating polarity growth via maintaining cellular calcium homeostasis. However, CchA may act differently as other components in calcium signaling pathway in Aspergillus nidulans. These findings provide an excellent opportunity to identify the potential pathway linking NDR protein-kinase network to calcium signaling pathway. (C) 2011 Elsevier Inc. All rights reserved.