Differential modulation of natural and adaptive immunity in Fischer rats exposed for 6 weeks to 60 Hz linear sinusoidal continuous-wave magnetic fields.
Differential modulation of natural and adaptive immunity in Fischer rats exposed for 6 weeks to 60 Hz linear sinusoidal continuous-wave magnetic fields.
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Fischer 大鼠暴露于 60 Hz 线性正弦连续波磁场 6 周，对自然和适应性免疫的差异调节。
DOI：
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发表时间：
1996
影响因子：
1.9
通讯作者：
Rosemonde Mandeville
中科院分区：
文献类型：
--
作者：
Lyna Tremblay;Michel Houde;Ginette Mercier;Jacky Gagnon;Rosemonde Mandeville
进行了两项独立的实验，以评估 60 Hz 线性极化、正弦、连续波磁场 (MF) 对在这些磁场下出生和长大的大鼠免疫系统性能的影响。每个实验持续6周。总共 96 只动物，分为每组 8 只动物，每天暴露于不同强度的 MF 下 20 小时，即假手术 (< 0.02 microT) 和 2、20、200 和 2000 microT。另一组动物被安置在一个单独的房间里，作为笼子对照（CC）。这些动物暴露于 < 0.02 microT 的环境 MF。在两个实验中评估了总 T 细胞和 B 细胞的以下免疫反应；脾淋巴细胞中 CD4+ 和 CD8+ 亚群以及自然杀伤 (NK) 细胞活性；腹膜巨噬细胞产生过氧化氢 (H2O2)、一氧化二氮 (NO) 和肿瘤坏死因子 (TNF)。我们的结果表明，暴露于 MF 6 周会导致 CD5+、CD4+ 和 CD8+ 群体数量显着减少。这些变化在暴露于 2000 microT 磁场的大鼠中更为显着。在暴露于 200 microT 磁场的动物中，也观察到 CD5+ 群体的减少虽然显着，但较低。线性回归分析证明了 MF 强度的剂量效应。在暴露于 20 和 200 microT 场的组中，B 淋巴细胞（Ig+ 细胞）群体也显示出 12% 的减少 (P < .05)。然而，这些结果并不显着，并且无法证明与 MF 强度的关系。相比之下，对脾脏 NK 细胞活性的评估显示，暴露于 2000 microT 场的动物的脾 NK 细胞活性增加了 50% (P < .05)。腹膜巨噬细胞中 TNF 活性和 NO 分泌的评估未获得显着结果。通过线性回归分析，佛波醇 12-肉豆蔻酸酯 13-乙酸酯 (PMA) 刺激的腹膜细胞的少数亚群的净 H2O2 产量显示出正的剂量反应相关性。综上所述，我们的结果表明，大鼠体内暴露于 60 Hz MF 6 周，可以以剂量依赖的方式对自然免疫和适应性免疫的效应细胞产生显着的免疫扰动。
Two separate, independent experiments were conducted to evaluate the effect of 60 Hz linearly polarized, sinusoidal, continuous-wave magnetic fields (MFs) on immune system performances in rats born and raised under these fields. Each experiment lasted for 6 weeks. A total of 96 animals, divided into groups of eight animals each, was exposed for 20 h/day to MFs of different intensities, i.e., sham (< 0.02 microT) and 2, 20, 200, and 2000 microT. Another group of animals, which was housed in a separate room, served as cage controls (CC). These animals were exposed to ambient MFs of < 0.02 microT. The following immune responses were evaluated in both experiments total T and B cells; CD4+ and CD8+ subpopulation and natural killer (NK) cell activity in splenic lymphocytes; hydrogen peroxide (H2O2), nitrous oxide (NO), and tumor necrosis factor (TNF) production by peritoneal macrophages. Our results show that a 6 week exposure to MFs induced a significant decrease in the number of CD5+, CD4+, and CD8+ populations. These changes were even more significant in rats that were exposed to fields of 2000 microT. A lower, although significant, decrease in the CD5+ population was also observed in animals that were exposed to fields of 200 microT. Linear regression analysis demonstrated a dose effect with MF intensity. B lymphocyte (Ig+ cell) populations also showed a 12% decrease (P < .05) in the groups that were exposed to fields of 20 and 200 microT. However, these results were not significant, and no relation with MF intensities could be demonstrated. In contrast, evaluation of splenic NK cell activity revealed a 50% increase (P < .05) in animals that were exposed to fields of 2000 microT. No significant results were obtained from the evaluation of TNF activity and NO secretion in peritoneal macrophages. Phorbol 12-myristate 13-acetate (PMA)-stimulated and net H2O2 productions for a minor subpopulation of peritoneal cells showed positive dose-response correlations by linear regression analysis. Taken together, our results suggest that an in vivo exposure of rats for 6 weeks to 60 Hz MFs can induce significant immunological perturbations on effector cells of both natural and adaptive immunity in a dose-dependent fashion.
影响因子：
4.4
作者：
D. Bass;J. W. Parce;L. Dechatelet;P. Szejda;M. Seeds;M. Thomas
通讯作者：
D. Bass;J. W. Parce;L. Dechatelet;P. Szejda;M. Seeds;M. Thomas
影响因子：
2.9
作者：
MARLETTA, MA;YOON, PS;WISHNOK, JS
通讯作者：
WISHNOK, JS