Simultaneous Determination of Six Triterpenoid Acids in Ganoderma by RP-HPLC
Simultaneous Determination of Six Triterpenoid Acids in Ganoderma by RP-HPLC
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发表时间：
2009
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通讯作者：
Su Jian-fen
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作者：
Su Jian-fen
目的建立同时测定灵芝中灵芝酸A、灵芝酸B、灵芝酸C2、灵芝酸G、灵芝酸E和灵芝酸A 6种三萜酸含量的反相高效液相色谱法（4.6 mm×250 mm，5 μm），采用乙腈-水梯度洗脱以0.8%乙酸为移动的流动相，流速1 mL·min-1，检测波长为254 nm，柱温为室温6种成分的平均回收率分别为100.06%、0.175 - 2.100、0.206 - 2.475、0.188 - 2.250、0.275 - 3.300、0.120 -1.440 μg（RSD=2.6%）、99.55%（RSD= 1.6%）、100.75%（RSD= 1.5%）、98.86%（RSD= 1.9%）、99.26%（RSD=2.3%）、98.95%（RSD=1.3%）。
OBJECTIVE To establish a RP-HPLC method for simultaneous determination of six kinds of triterpenoid acids in Ganoderma,i.e.ganoderic acid A,ganoderic acid B,ganoderic acid C2,ganoderic acid G,ganoderic acid E and lucidenic acid A.METHODS The separation was performed on a Diamonsil-C18 column(4.6 mm×250 mm,5 μm),using a gradient elution with acetonitrile-wate(rcontaining 0.8% acetic acid)as the mobile phase.The flow rate was 1 mL·min-1,the detection wavelength was at 254 nm and the temperature of column was at room temperature.RESULTS The linear relationship were obtained within the range of 0.650-7.800,0.175-2.100,0.206-2.475,0.188-2.250,0.275-3.300,0.120-1.440 μg for the six components respectively.The average recoveries of the six components(n=6) were 100.06%(RSD=2.6%),99.55 %(RSD=1.6 %),100.75%(RSD=1.5 %),98.86 %(RSD=1.9 %),99.26%(RSD=2.3%) and 98.95%(RSD=1.3%).CONCLUSION The simple,rapid and reliable method could be used for the quality control of Ganoderma.