In situ printing of scaffolds for reconstruction of bone defects.
In situ printing of scaffolds for reconstruction of bone defects.
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DOI：
10.1016/j.actbio.2021.03.009
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发表时间：
2021-03
影响因子：
9.7
通讯作者：
Azadeh Mostafavi;Tuerdimaimaiti Abudula;Carina S. Russell;E. Mostafavi;Tyrell J Williams;N. Salah;A. Alshahrie;Seth P Harris;Seyed Masoud Moosavi Basri;Y. Mishra;T. Webster;Adnan Memić;A. Tamayol
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作者：
Azadeh Mostafavi;Tuerdimaimaiti Abudula;Carina S. Russell;E. Mostafavi;Tyrell J Williams;N. Salah;A. Alshahrie;Seth P Harris;Seyed Masoud Moosavi Basri;Y. Mishra;T. Webster;Adnan Memić;A. Tamayol
骨缺损通常是由外伤和肿瘤切除引起的，严重尺寸的缺损会压倒天然组织的再生能力。事实证明，自体、异种和同种异体移植等修复策略不足以重建和再生这些缺陷。我们首次介绍了手持式熔纺三维打印机的使用，该打印机可以将材料直接沉积在缺陷部位内，以正确填充空腔并形成独立式支架。工程复合材料长丝由掺杂氧化锌纳米粒子和羟基磷灰石微粒的聚己内酯（PCL）生成。使用 PCL 材料可以进行低温打印，避免周围组织过热。原位打印的支架对湿骨组织表现出中等的粘附力，可以防止支架脱位。打印的支架具有骨传导性，支持间充质干细胞的骨分化。小鼠皮下体内打印支架的生物相容性显示出有希望的结果。重要性声明•骨缺损很常见，在许多情况下，从立即干预中受益。•引入了便携式手持式打印机，可以将硬质聚合物材料直接打印到缺损部位，从而重建缺损组织的几何特征。•在体外和体内都证明了所研究设备的易用性。•原位打印的支架粘附在组织上并支持生长和培养干细胞的分化。它们还可以防止细菌生长，而不会引起过度炎症。
Bone defects are commonly caused by traumatic injuries and tumor removal and critically sized defects overwhelm the regenerative capacity of the native tissue. Reparative strategies such as auto, xeno, and allografts have proven to be insufficient to reconstruct and regenerate these defects. For the first time, we introduce the use of handheld melt spun three dimensional printers that can deposit materials directly within the defect site to properly fill the cavity and form free-standing scaffolds. Engineered composite filaments were generated from poly(caprolactone) (PCL) doped with zinc oxide nanoparticles and hydroxyapatite microparticles. The use of PCL-based materials allowed low-temperature printing to avoid overheating of the surrounding tissues. Thein situprinted scaffolds showed moderate adhesion to wet bone tissue, which can prevent scaffold dislocation. The printed scaffolds showed to be osteoconductive and supported the osteodifferentiation of mesenchymal stem cells. Biocompatibility of the scaffolds uponin vivoprinting subcutaneously in mice showed promising results.Statement of significance•Bone defects are frequent and, in many cases, benefit from an immediate intervention.•A portable and handheld printer is introduced that allows the printing of hard polymeric materials directly into the defect site that allow reconstruction of the geometrical features of the defected tissue.•The ease-of-use of the investigated device was demonstrated both in vitro and in vivo.•The in situ printed scaffolds adhered to the tissue and supported the growth and differentiation of cultured stem cells. They also prevented bacterial growth without inducing excessive inflammation.