Association between an aggrecan gene polymorphism and lumbar disc degeneration
Association between an aggrecan gene polymorphism and lumbar disc degeneration
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DOI：
10.1097/00007632-199912010-00006
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发表时间：
1999-12-01
期刊：
影响因子：
3
通讯作者：
Kimura, T
中科院分区：
文献类型：
--
作者：
Kawaguchi, Y;Osada, R;Kimura, T
设计应用磁共振成像和聚合酶链反应研究聚集蛋白聚糖基因多态性与腰椎间盘退变的关系。分析聚集蛋白聚糖基因多态性与腰椎间盘疾病的关系。有研究表明，遗传因素或家族易感性有助于腰椎间盘突出症的发展。然而，与椎间盘疾病相关的确切遗传成分仍不清楚。最近，在人聚集蛋白聚糖基因的区域中已经鉴定出多态性。在高度保守的重复序列区域中存在可变数目的串联重复序列多态性。参与者是64名有或没有腰部问题的年轻女性。磁共振成像用于评估椎间盘退变和突出。从所有参与者中提取基因组脱氧核糖核酸。聚合酶链反应检测聚集蛋白聚糖基因的等位基因。分析聚集蛋白聚糖基因等位基因的分布与椎间盘退变和突出的关系。研究结果显示，在多水平椎间盘退变受试者中，具有少量重复的等位基因比例过高，从而表明存在显着的分布差异。等位基因的分布与椎间盘退变的严重程度也有显著性差异。任何等位基因之间没有显着的关联，无论是在numberer类型的椎间盘herniation.Conclusions：目前的研究表明，多层次和严重的椎间盘退变是存在于参与者与较短的可变数目的串联重复长度的聚集蛋白聚糖基因。这表明具有较短可变数目的串联重复长度的受试者。聚集蛋白聚糖基因具有在早期发展多水平椎间盘变性风险。
Design. A case-control study using magnetic resonance imaging findings and a polymerase chain reaction assay to investigate the association between aggrecan gene polymorphism and lumbar disc degeneration.Objective. To analyze whether the aggrecan gene polymorphism is related to lumbar disc disease in youngSummary of Background Data. It has been suggested,that agenetic factor or familial predisposition contributes to the development of lumbar disc herniation. However, the precise genetic component related to disc disease remains unclear. Recently, a polymorphism has been identified in the region of the human aggrecan gene. The expressed variable numbers of tandem repeat polymorphism occur in the highly conserved repeat region.Methods. The participants were 64 young women with or without low back problems. Magnetic resonance imaging was used to evaluate the degeneration and herniation of the intervertebral disc. Genomic deoxyribonucleic acid was extracted from all participants. A,polymerase chain reaction assay was carried out to detect the alleles of the aggrecan gene. The association of intervertebral disc degeneration and herniation with the distribution of the aggrecan gene alleles was analyzed.Results. Findings showed an overrepresentation of alleles with small numbers of repeats in subjects with multilevel disc degeneration, thus indicating a significant distribution difference. There also was a significant difference between the distribution of alleles and the severity of disc degeneration. No significant association was found between any of the alleles either in numberer type of disc herniation.Conclusions: The current study showed that multilevel and severe disc degeneration was present in the participants with shorter variable numbers of tandem repeat length of the aggrecan gene. This suggests that subjects with shorter variable numbers of tandem repeat length of.. the aggrecan gene have a risk of having multilevel disc degeneration develop at an early age.