Survival of retinal ganglion cells after transection of the optic nerve in adult cats: A quantitative study within two weeks
Survival of retinal ganglion cells after transection of the optic nerve in adult cats: A quantitative study within two weeks
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DOI：
10.1017/s0952523801181137
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发表时间：
2001-01
影响因子：
1.9
通讯作者：
M. Watanabe;N. Inukai;Y. Fukuda
中科院分区：
文献类型：
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作者：
M. Watanabe;N. Inukai;Y. Fukuda
我们以前曾报道，成年猫的少数视网膜神经节细胞（RGC）在视神经（ON）横断后存活2个月，并且α细胞在不同类型的RGC中具有最大的存活能力（Watanabe et al.，1995年）。在这里，我们报告的时间过程中，RGC的生存后15天内，ON横断使用逆行标记DiI注射到猫的双侧外侧膝状体核。DiI标记的RGCs在视网膜中央以及周边的密度没有变化，直到第3天后ON横断，然后迅速下降，到43%的原始密度在第7天，下降到19%的第14天。然后，我们将荧光黄细胞内注射到DiI标记的RGCs中，以检查存活的α和β细胞之间的时间过程差异。与总存活RGC的密度变化相似，存活β细胞的比例直到第3天才发生变化，然后在第4天迅速降至原始密度的65%，到第14天降至12%。相比之下，64%的α细胞存活14天后轴突切断。对存活时间的回归线分析表明，β细胞在切断后第3 ~ 7天有一个快速的死亡期，而α细胞则没有，存活的β细胞在切断后第3天开始在神经纤维层中出现轴突样出芽，以后更为明显。
We have previously reported that a small number of retinal ganglion cells (RGCs) of adult cats survive 2 months after transection of the optic nerve (ON) and that α cells have the greatest ability to survive among different types of RGCs (Watanabe et al., 1995). Here we report the time course of RGC survival within 15 days after ON transection using retrograde labeling with DiI injected into the bilateral lateral geniculate nuclei of cats. The density of DiI-labeled RGCs in the central retina as well as in the periphery did not change until day 3 after ON transection, then decreased rapidly, to 43% of the original density on day 7, and falling to 19% by day 14. We then intracellularly injected Lucifer yellow into the DiI-labeled RGCs to examine the difference in the time course between surviving α and β cells. Similar to the density change in total surviving RGCs, the proportion of surviving β cells did not change until day 3, then decreased rapidly to 65% of the original density on day 4, falling to 12% by day 14. By contrast, 64% of α cells survived for 14 days after axotomy. Analysis of regression lines for survival time courses indicated that death of β cells was characterized with a rapid period phase from day 3 to day 7 after axotomy whereas that of α cells lacked it. Axon-like sprouting from surviving β cells was first recognized in the nerve fiber layer on day 3, and were later more conspicuous.