LACK OF EVIDENCE FOR INVOLVEMENT OF KNOWN HUMAN RETROVIRUSES IN MULTIPLE-SCLEROSIS
LACK OF EVIDENCE FOR INVOLVEMENT OF KNOWN HUMAN RETROVIRUSES IN MULTIPLE-SCLEROSIS
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DOI：
10.1038/322177a0
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发表时间：
1986-07-10
期刊：
影响因子：
64.8
通讯作者：
POSER, S
中科院分区：
文献类型：
--
作者：
KARPAS, A;KAMPF, U;POSER, S
Koprowskiet等人最近报道的人类t细胞嗜淋巴逆转录病毒（htlv）可能参与多发性硬化症（MS）的发展引起了极大的兴趣。该报告主要基于免疫学证据，使用病毒抗原或破坏病毒粒子的酶联免疫吸附测定（elisa）。因此，我们通过筛选MS患者的血清和脑脊液（CSF）样本，分别针对日本细胞（MT-1和MT-2系）感染成人t细胞白血病（ATL）病毒（ATLV/HTLV-I）的细胞系、我们自己从英国黑人ATL患者中分离出来的细胞系、产生HTLV-II的MoT细胞系和我们自己含有当地获得性免疫缺陷综合征（AIDS）病毒（C-LAV/HTLV-III）的t细胞系进行了确认。我们未能在血清或脑脊液中找到针对这些逆转录病毒的抗体。此外，两种病毒都无法从两名MS患者的外周血中分离出来。
The recent report by Koprowskiet al.1that human T-cell lymphotropic retroviruses (HTLVs) may be involved in the development of multiple sclerosis (MS) has aroused much interest2. The report was based largely on immunological evidence, using enzyme-linked immunosorbent assays (ELISAs) with viral antigens or disrupted virions. We have accordingly sought confirmation by screening sera and cerebrospinal fluid (CSF) samples from MS patients against cell lines infected respectively with adult T-cell leukaemia (ATL) virus (ATLV/HTLV-I) of Japanese cells (MT-1 and MT-2 lines), our own isolate from British black patients with ATL, the MoT cell line which produce HTLV-II, and our own T-cell line containing a local isolate of acquired immune deficiency syndrome (AIDS) virus (C-LAV/HTLV-III). We have failed to find antibodies against these retroviruses in the sera or CSF. Furthermore, neither virus could be isolated from the peripheral white blood cells of two MS patients.