Determination of the survival of bacteriophage M13 from chemical and physical challenges to assist in its sustainable bioprocessing
Determination of the survival of bacteriophage M13 from chemical and physical challenges to assist in its sustainable bioprocessing
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DOI：
10.1007/s12257-012-0776-9
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发表时间：
2013-06-01
影响因子：
3.2
通讯作者：
Keshavarz-Moore, Eli
中科院分区：
文献类型：
--
作者：
Branston, Steven D.;Stanley, Emma C.;Keshavarz-Moore, Eli
噬菌体是天然的具有感染性的颗粒，在其细菌宿主中极其高效地复制。因此，处理细菌菌株生物制品的设施通常会专注于避免噬菌体污染。然而，噬菌体本身作为一类全新的工业试剂，如基于生物的纳米材料、传递载体和抗菌剂，现在显示出巨大的前景。因此，这对它们的大规模生产提出了新的挑战，可能是在与宿主生物体共享的合同设施中。关键问题是，面对物理和化学挑战时，对单个噬菌体行为的了解往往是不完整的，这使得将其安全引入设施的决策复杂化。这项研究针对丝状噬菌体M13解决了这个问题。结果发现，确定有效去污剂的实验很重要：三种试验中有两种是无效的。维康被认为是首选的消毒剂。噬菌体M13被证实是高度耐干燥的，半衰期长达120天。相反，它在强酸性和碱性条件下以及95摄氏度以上的温度下完全失活。通过了解噬菌体对这些挑战的反应，可以实现可持续生产的步骤。
Bacteriophages are naturally infectious particles that replicate extremely efficiently in their bacterial hosts. Consequently, a facility processing bioproducts from a bacterial strain would be typically expected to focus on avoiding bacteriophage contamination. However, bacteriophages themselves are now showing great promise as a whole new class of industrial agents, such as biologically based nano-materials, delivery vectors and antimicrobials. This therefore raises a new challenge for their large-scale manufacture, potentially in contracted facilities shared with the host organism. The key issue is that knowledge of individual bacteriophage behaviour in the face of physical and chemical challenges is frequently incomplete, complicating decision-making regarding their safe introduction to a facility. This study tackles this issue for the filamentous bacteriophage M13. It was found that experimentation to determine an effective decontamination agent was important: Two of the three tested were ineffective. Virkon was considered to be the disinfectant of choice. Bacteriophage M13 was confirmed to be highly desiccation resistant, exhibiting a half-life of up to 120 days. Conversely, it was completely inactivated by strongly acidic and alkaline conditions and by temperatures above 95A degrees C. By understanding the response of a bacteriophage to these challenges, steps towards their sustainable manufacture can be achieved.