Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation.
Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation.
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DOI：
10.1002/ehf2.12123
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发表时间：
2017-05
影响因子：
3.8
通讯作者：
Ono K
中科院分区：
文献类型：
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作者：
Koyama S;Kuragaichi T;Sato Y;Kuwabara Y;Usami S;Horie T;Baba O;Hakuno D;Nakashima Y;Nishino T;Nishiga M;Nakao T;Arai H;Kimura T;Ono K
最近的研究表明，血清microRNA（miR）丰度对心力衰竭的诊断或预后提供信息。然而，miR在急性心力衰竭中的动力学和动力学在很大程度上是未知的。对个体沿着其治疗过程的血清miR变化进行系列测量和分析可以减少个体间差异，并且应该检测与疾病机制相关的潜在重要血清miR。基于这一概念，我们分析了在入院当天和住院第7天连续获得的血液样品的血清miR特征。这项前瞻性、观察性研究纳入了42例连续急性心力衰竭患者（74 ± 1岁，24例男性）。从入院到第7天，大多数患者显示临床改善。在这样的队列中，我们通过两种不同的筛选方法（定量PCR和高通量测序）检测到血清miR的几种波动。其中一种波动性血清miR-122 - 5 p从第1天至第7天显著降低[中位任意单位（第1：第3分位数值）; 4.62 [2.39：12.3]至3.07 [1.67：5.39]，P = 0.007]。这种波动与血清肝功能标志物的变化显著相关（估计系数和95%置信区间;对天冬氨酸转氨酶变化1.69，0.890-2.484，P < 0.001和r = 0.560;对丙氨酸转氨酶变化1.09，0.406-1.771，P = 0.007和r = 0.428）。急性心力衰竭患者的血清miR特征可能指示疾病的严重程度或患者对治疗干预的反应。值得注意的是，血清miR-122 - 5 p水平反映了这种情况下的肝损伤。
Recent studies have shown that serum microRNA (miR) abundance is informative for the diagnosis or prognosis of heart failure. However, the dynamics and kinetics of miRs in acute heart failure are largely unknown. Serial measurement and analysis of serum miRs changes in individuals along their therapeutic course could reduce inter‐individual variation and should detect potentially important serum miRs related to disease mechanisms. Based on this concept, we profiled serum miR signatures of blood samples that were obtained sequentially on the day of admission and on hospital Day 7. This prospective, observational study included 42 consecutive acute heart failure patients (74 ± 1 years old, 24 male). From admission to Day 7, most of the patients showed clinical improvement. In such a cohort, we detected several fluctuations of serum miRs by two distinct screening methods (quantitative PCR and high‐throughput sequencing). One of these fluctuating serum miRs, miR‐122‐5p, decreased significantly from Day 1 to Day 7 [median arbitrary unit (1st:3rd quantile value); 4.62 [2.39:12.3] to 3.07 [1.67:5.39], P = 0.007]. This fluctuation was significantly correlated with changes in serum liver function markers (estimated coefficient and 95% confidence interval; vs change in aspartate aminotransferase 1.69, 0.890–2.484, P < 0.001 and r = 0.560, vs change in alanine aminotransferase 1.09, 0.406–1.771, P = 0.007 and r = 0.428). The serum miR signature of patients with acute heart failure might indicate the severity of the disease or patients' response to therapeutic intervention. Notably, serum miR‐122‐5p levels reflect liver damage in this condition.