Influence of lineage-specific cytokines on commitment and asymmetric cell division of haematopoietic progenitor cells.
Influence of lineage-specific cytokines on commitment and asymmetric cell division of haematopoietic progenitor cells.
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谱系特异性细胞因子对造血祖细胞的定型和不对称细胞分裂的影响。
DOI：
10.1046/j.1365-2141.2002.03638.x
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发表时间：
2002
影响因子：
6.5
通讯作者：
Rodgers,GriffinP
中科院分区：
文献类型：
--
作者：
Chen,Ling;Zhang,Jiachang;Tang,DeliaC;Fibach,Eitan;Rodgers,GriffinP
我们研究了在原代和传代培养期间细胞因子对AC 133+细胞的红系和髓系发育的影响。在含有促红细胞生成素（EPO）的液体培养基中培养14天的细胞扩增831倍，红系细胞占98.2%。暴露于粒细胞集落刺激因子（G-CSF）的类似培养物生长了1350倍，骨髓细胞占97.4%。为了评估具有EPO诱导的细胞是否可以在此时响应于G-CSF信号，或反之亦然，在再培养14天后，用G-CSF再生长EPO刺激的群体，其构成5075倍的骨髓细胞的95.2%。 相反，用EPO再培养G-CSF刺激的群体导致4083倍的生长，其中红系细胞为81.4%。EPO或G-CSF的半固体培养表明，培养14 d后，部分细胞集落具有自我更新的能力，并可被诱导分化为不同的细胞系。 原始标志物CD 34和Notch 1或谱系标志物EPO-R和CD 13的分析，单细胞逆转录聚合酶链反应显示，2- 16个细胞含有至少一个表达Notch 1的CD 34阳性细胞，并且在CD 34阳性或CD 34阴性细胞上出现EPO-R和CD 13的共表达。从单细胞数据证实了不对称细胞分裂和共表达。该研究为分析多潜能祖细胞发育提供了一个有用的模型，并表明祖细胞在定型前共表达来自不同谱系途径的基因，细胞因子影响谱系定型。
We examined the influence of cytokines on erythroid‐ and myeloid‐lineage development of AC133+cells during primary and secondary cultures. Cells cultured for 14 d in liquid medium containing erythropoietin (EPO) were amplified 831‐fold with 98·2% erythroid cells. A similar culture exposed to granulocyte colony‐stimulating factor (G‐CSF) grew 1350‐fold with 97·4% myeloid cells. To assess whether the cells with EPO inducement could respond at this point to G‐CSF signal, or vice versa, the EPO‐stimulated population was re‐grown with G‐CSF, constituting 95·2% myeloid, of 5075‐fold, cells after 14 d of re‐culture. Conversely, reculture of the G‐CSF‐stimulated population with EPO resulted in a 4083‐fold growth with 81·4% erythroid cells. Semisolid culture containing EPO orG‐CSF showed that some individual colonies had self‐ renewal potential after 14 d culture and could be induced todevelop into a different lineage. Analysis of primitive markers, CD34 and Notch1, or lineage markers, EPO‐R and CD13, by single‐cell reverse transcription polymerase chain reaction showed that individual colonies of 2–16 cells contained at least one CD34‐positive cell with expression ofNotch1 and co‐expression of EPO‐R and CD13 appeared on either CD34‐positive or CD34‐negative cells.In situhybridization with the same cell surface markers in cell populations confirmed the asymmetric cell division and co‐expression from single cell data. The study provides a useful model for the analysis of multipotential progenitor development, and indicates that progenitor cells co‐express genes from different lineage pathways before commitment and that cytokines influence lineage commitment.