Discovery and characterization of two new stem rust resistance genes in Aegilops sharonensis.
Discovery and characterization of two new stem rust resistance genes in Aegilops sharonensis.
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DOI：
10.1007/s00122-017-2882-8
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发表时间：
2017-06
期刊：
影响因子：
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通讯作者：
Wulff BBH
中科院分区：
文献类型：
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作者：
Yu G;Champouret N;Steuernagel B;Olivera PD;Simmons J;Williams C;Johnson R;Moscou MJ;Hernández-Pinzón I;Green P;Sela H;Millet E;Jones JDG;Ward ER;Steffenson BJ;Wulff BBH
在沙龙盾蚧中鉴定出两个新的小麦茎锈病抗性基因Sr-1644-1Sh和Sr-1644-5Sh，它们对具有广泛毒力的小麦茎锈病非洲小种有效。茎锈病是世界上最重要的小麦病害之一。当单茎抗锈病基因在小麦中部署时，它们通常会迅速被病原体克服。为此，我们开始在不同的小麦亲缘种中寻找新的抗性来源，并确定野生山羊草种Aegilops sharonesis （Sharon goatgrass）是小麦茎锈病抗性的丰富储存库。本研究的目的是在伊蚊中发现和定位新的Sr基因。并探讨利用全基因组关联研究（GWAS）鉴定Sr新基因的可能性。我们在抗性和易感材料之间建立了两个双亲本种群。并进行QTL和连锁分析。在一个F6重组自交系和一个F2群体中，鉴定出两个基因分别位于染色体1Ssh的短臂上，标记为Sr-1644-1Sh和染色体5Ssh的长臂上，标记为Sr-1644-5Sh。基因Sr-1644-1Sh对TTKSK （Ug99人种组的一员）具有高水平的抗性，而基因Sr-1644-5Sh对TRTTF（在也门发现的另一种广泛致命的种族）具有强抗性。此外，还对125种不同的Ae进行了GWAS。沙龙种质的茎锈病抗性。GWAS检测到Sr-1644-1Sh基因，未检测到Sr-1644-5Sh基因，说明GWAS的有效性可能受到标记密度、群体结构、低等位基因频率等因素的影响。本文的在线版本（doi:10.1007/s00122-017-2882-8）包含补充材料，仅供授权用户使用。
We identified two novel wheat stem rust resistance genes, Sr-1644-1Sh and Sr-1644-5Sh in Aegilops sharonensis that are effective against widely virulent African races of the wheat stem rust pathogen. Stem rust is one of the most important diseases of wheat in the world. When single stem rust resistance (Sr) genes are deployed in wheat, they are often rapidly overcome by the pathogen. To this end, we initiated a search for novel sources of resistance in diverse wheat relatives and identified the wild goatgrass species Aegilops sharonesis (Sharon goatgrass) as a rich reservoir of resistance to wheat stem rust. The objectives of this study were to discover and map novel Sr genes in Ae. sharonensis and to explore the possibility of identifying new Sr genes by genome-wide association study (GWAS). We developed two biparental populations between resistant and susceptible accessions of Ae. sharonensis and performed QTL and linkage analysis. In an F6 recombinant inbred line and an F2 population, two genes were identified that mapped to the short arm of chromosome 1Ssh, designated as Sr-1644-1Sh, and the long arm of chromosome 5Ssh, designated as Sr-1644-5Sh. The gene Sr-1644-1Sh confers a high level of resistance to race TTKSK (a member of the Ug99 race group), while the gene Sr-1644-5Sh conditions strong resistance to TRTTF, another widely virulent race found in Yemen. Additionally, GWAS was conducted on 125 diverse Ae. sharonensis accessions for stem rust resistance. The gene Sr-1644-1Sh was detected by GWAS, while Sr-1644-5Sh was not detected, indicating that the effectiveness of GWAS might be affected by marker density, population structure, low allele frequency and other factors. The online version of this article (doi:10.1007/s00122-017-2882-8) contains supplementary material, which is available to authorized users.