Hepatitis C and HIV detection by blood RNA-sequencing in cohort of smokers.
Hepatitis C and HIV detection by blood RNA-sequencing in cohort of smokers.
复制标题
DOI：
10.1038/s41598-023-28156-4
复制
发表时间：
2023-01-24
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
通过RNA和DNA测序检测病毒提高了对人类病毒组的理解。我们试图通过在一个大型研究队列中二次使用RNA测序（RNA-seq）数据来识别血液病毒特征。在具有可用测序数据的研究受试者中揭示具有公共卫生意义的未诊断感染的能力可以使流行病学调查成为可能，并可能导致诊断和治疗干预，在临床背景下利用现有的研究数据。我们在来自COPD富集的当前和既往吸烟者人群的外周血RNA-seq数据中检测到病毒RNA。评价了病毒检测与报告的感染和相关疾病结局之间的相关性。我们在228名受试者中鉴定出丙型肝炎病毒RNA，在30名受试者中鉴定出HIV RNA。总体而言，我们观察到31种病毒，包括EB病毒和巨细胞病毒。我们观察到丙型肝炎和HIV感染分别在报告肝病和HIV感染的受试者中富集。在丙型肝炎和HIV感染的受试者中观察到更高的干扰素表达评分。通过从当前和以前的吸烟者队列中二次使用RNA-seq，我们检测了外周血病毒特征。我们确定了艾滋病毒和丙型肝炎病毒（HCV），强调了本研究所述方法的潜在公共卫生影响。我们观察到报告的感染，慢性感染结果和宿主转录组反应的相关性，提供证据支持该方法的有效性。
Detection of viruses by RNA and DNA sequencing has improved the understanding of the human virome. We sought to identify blood viral signatures through secondary use of RNA-sequencing (RNA-seq) data in a large study cohort. The ability to reveal undiagnosed infections with public health implications among study subjects with available sequencing data could enable epidemiologic surveys and may lead to diagnosis and therapeutic interventions, leveraging existing research data in a clinical context. We detected viral RNA in peripheral blood RNA-seq data from a COPD-enriched population of current and former smokers. Correlation between viral detection and both reported infections and relevant disease outcomes was evaluated. We identified Hepatitis C virus RNA in 228 subjects and HIV RNA in 30 subjects. Overall, we observed 31 viral species, including Epstein-Barr virus and Cytomegalovirus. We observed an enrichment of Hepatitis C and HIV infections among subjects reporting liver disease and HIV infections, respectively. Higher interferon expression scores were observed in the subjects with Hepatitis C and HIV infections. Through secondary use of RNA-seq from a cohort of current and former smokers, we detected peripheral blood viral signatures. We identified HIV and Hepatitis C virus (HCV), highlighting potential public health implications for the approach described this study. We observed correlations with reported infections, chronic infection outcomes and the host transcriptomic response, providing evidence to support the validity of the approach.
登录
查看更多内容
影响因子：
2.7
作者：
Parker MM;Chase RP;Lamb A;Reyes A;Saferali A;Yun JH;Himes BE;Silverman EK;Hersh CP;Castaldi PJ
通讯作者：
Castaldi PJ
影响因子：
3.7
作者：
Putcha N;Puhan MA;Drummond MB;Han MK;Regan EA;Hanania NA;Martinez CH;Foreman M;Bhatt SP;Make B;Ramsdell J;DeMeo DL;Barr RG;Rennard SI;Martinez F;Silverman EK;Crapo J;Wise RA;Hansel NN
通讯作者：
Hansel NN
影响因子：
6.4
作者：
Ockenhouse, CF;Bernstein, WB;Vahey, MT
通讯作者：
Vahey, MT
影响因子：
25.7
作者：
Westbrook, Rachel H.;Dusheiko, Geoffrey
通讯作者：
Dusheiko, Geoffrey
当前和前吸烟者中的外周血微生物特征。
影响因子：
4.6
作者：
Morrow JD;Castaldi PJ;Chase RP;Yun JH;Lee S;Liu YY;Hersh CP
通讯作者：
Hersh CP