Thermodynamics of ribonuclease T1 denaturation.
Thermodynamics of ribonuclease T1 denaturation.
复制标题
核糖核酸酶 T1 变性的热力学。
DOI：
10.1021/bi00135a019
复制
发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Pace,CN
中科院分区：
文献类型：
--
作者：
Hu,CQ;Sturtevant,JM;Thomson,JA;Erickson,RE;Pace,CN
耶鲁大学化学系和分子生物物理和生物化学系，康涅狄格州纽黑文，06511，和医学生物化学和遗传学系，得克萨斯州A&M大学，学院站，得克萨斯州77843，收到1991年12月23日；修订手稿收到1992年3月11日摘要：差示扫描量热法已被用来研究核糖核酸酶TL的变性热力学作为pH范围2-10的函数，以及作为氯化钠和氯化镁浓度的函数。在pH=7的30 mm管子缓冲液中，其热力学参数为：熔融温度Tx~2=48.9±0.1℃，热变H=95.5±0.9千卡·摩尔~(-1)，热容变化，
Department of Chemistry and Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511, and Department of Medical Biochemistry and Genetics and Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843 Received December 23, 1991; Revised Manuscript Received March 11, 1992 abstract: Differential scanning calorimetry has been used to investigate the thermodynamics of denaturation of ribonuclease Tl as a function of pH over the pH range 2-10, and as a function of NaCl and MgCl2 concentration. At pH 7 in 30 mM PIPES buffer, the thermodynamic parameters are as follows: melting temperature, Tx¡ 2= 48.9±0.1 C; enthalpy change, AH= 95.5±0.9 kcal mol-1; heat capacity change,