Identification of a fourth class of proteins linked to the murine major histocompatibility complex.
Identification of a fourth class of proteins linked to the murine major histocompatibility complex.
复制标题
鉴定与鼠主要组织相容性复合体相关的第四类蛋白质。
DOI：
10.1073/pnas.79.9.3001
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发表时间：
1982
影响因子：
11.1
通讯作者：
McDevitt,HO
中科院分区：
文献类型：
--
作者：
Monaco,JJ;McDevitt,HO
一系列的蛋白质，生物化学和遗传学上不同于以前定义的小鼠主要组织相容性复合体I类和II类抗原沉淀的同源抗H-2d抗血清。已经确定了16种这样的蛋白质，它们具有一定的分子量（约15，000 - 30，000）和等电点（pI约4-9）。这些蛋白质不是糖基化的，并且它们可能不在细胞表面表达。它们在正常巨噬细胞和巨噬细胞系中表达最强，并且也在成纤维细胞、B、T和空细胞系中发现。控制这些蛋白质表达的基因已被初步定位在H-2复合物中，位于K和I-A亚区之间。已经定义了三个等位基因：H-2单倍型B和q的小鼠具有“无效”等位基因，即，不表达任何可证实的蛋白质产物。d单倍型小鼠可通过其二维凝胶图谱与迄今为止测试的所有其它阳性H-2型小鼠（a、k、f、s和ja）区分开。
A series of proteins biochemically and genetically distinct from previously defined murine major histocompatibility complex class I and class II antigens is precipitated by a congeneic anti-H-2d antiserum. Sixteen such proteins have been defined, exhibiting a range of molecular weights (approximately 15,000-30,000) and isoelectric points (pI approximately 4-9). These proteins are not glycosylated, and they are probably not expressed at the cell surface. They are expressed most strongly in normal macrophages and macrophage cell lines and are also found in fibroblasts, B, T, and null cell lines. The genes controlling the expression of these proteins have been tentatively mapped within the H-2 complex, between the K and I-A subregions. Three alleles have been defined: mice of the H-2 haplotypes b and q possess a "null" allele, i.e., do not express any demonstrable protein product. Mice of the d haplotype cane be distinguished by their two-dimensional gel pattern from mice of all other positive H-2 types tested thus far (a, k, f, s, and ja).