Bombesin functionalized 64Cu-copper sulfide nanoparticles for targeted imaging of orthotopic prostate cancer
Bombesin functionalized 64Cu-copper sulfide nanoparticles for targeted imaging of orthotopic prostate cancer
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Bombesin 功能化 64Cu-硫化铜纳米粒子用于原位前列腺癌的靶向成像
DOI：
10.2217/nnm-2018-0062
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发表时间：
2018-07-01
期刊：
影响因子：
5.5
通讯作者：
Ai, Hua
中科院分区：
文献类型：
--
作者：
Cai, Huawei;Xie, Fang;Ai, Hua
目的：合成Bom-PEG-[Cu-64] CuS纳米粒子（NPs），并评价其在前列腺肿瘤原位显像中的应用价值。材料与方法：在水溶液中通过（CuCl 2）-Cu-64和Na 2S反应合成[Cu-64] CuS纳米粒子。然后将具有或不具有蛙皮素肽的PEG接头缀合至[Cu-64] CuS NP的表面以产生Bom-PEG-[Cu-64] CuS和PEG-[Cu-64] CuS NP。将这两种纳米颗粒用于体外前列腺癌细胞的特异性摄取测试和体内原位前列腺肿瘤的成像。结果：成功合成了Bom-PEG-[Cu-64] CuS和PEG-[Cu-64] CuS纳米粒，核直径约为5 nm。放射性细胞摄取显示Bom-PEG-[Cu-64] CuS能够特异性结合前列腺癌细胞，microPET-CT成像显示原位前列腺肿瘤的清晰可视化。结论：放射性标记的Bom-PEG-[Cu-64] CuS纳米粒具有作为microPET-CT原位前列腺肿瘤成像的理想药物的潜力。
Aim: To synthesize and evaluate the imaging potential of Bom-PEG-[Cu-64] CuS nanoparticles (NPs) in orothotopic prostate tumor. Materials & methods: [Cu-64] CuS NPs were synthesized in aqueous solution by (CuCl2)-Cu-64 and Na2S reaction. Then PEG linker with or without bombesin peptide were conjugated to the surface of [Cu-64] CuS NPs to produce Bom-PEG-[Cu-64] CuS and PEG-[Cu-64] CuS NPs. These two kinds of NPs were used for testing specific uptake in prostate cancer cells in vitro and imaging of orthotopic prostate tumor in vivo. Results: Bom-PEG-[Cu-64] CuS and PEG-[Cu-64] CuS NPs were successfully synthesized with core diameter of approximately 5 nm. Radioactive cellular uptake revealed that Bom-PEG-[Cu-64] CuS was able to specifically bind to prostate cancer cells, and the microPET-CT imaging indicated clear visualization of orthotopic prostate tumors. Conclusion: Radiolabeled Bom-PEG-[Cu-64] CuS NPs have potential as an ideal agent for orthotopic prostate tumor imaging by microPET-CT.