Re-evaluating the p7 viroporin structure.
Re-evaluating the p7 viroporin structure.
复制标题
重新评估 p7 病毒孔蛋白结构。
DOI：
10.1038/s41586-018-0561-9
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发表时间：
2018
期刊：
影响因子：
64.8
通讯作者：
Oestringer BP
中科院分区：
文献类型：
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作者：
Oestringer BP
方法根据核磁共振弛豫数据估算配合物的大小。为了排除内部运动快于或慢于整体翻滚时间的残留物的数据，旋转相关时间τc由15 N弛豫速率的20%修剪平均值计算，R1和R2分别为1.34 s-1和14.80 s-1。使用基于HSQC的实验测量15 N-弛豫速率。使用斯托克斯定律从τc计算分子质量，假设水化壳为
MethodsEstimation of complex size from NMR relaxation data. To exclude data from residues with internal motions faster or slower than the overall tumbling time, the rotational correlation time τc was calculated from the 20% trimmed means of the 15N-relaxation rates 16, which were 1.34 s− 1 and 14.80 s− 1 for R1 and R2, respectively. 15N-relaxation rates were measured using HSQC-based experiments. The molecular mass was calculated from τc using Stokes’ law, assuming a hydration shell of