Receptors for interleukin-3 (IL-3) and growth hormone mediate an IL-6-type transcriptional induction in the presence of JAK2 or STAT3.
Receptors for interleukin-3 (IL-3) and growth hormone mediate an IL-6-type transcriptional induction in the presence of JAK2 or STAT3.
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DOI：
10.1182/blood.v86.5.1671.bloodjournal8651671
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发表时间：
1995-09
期刊：
影响因子：
20.3
通讯作者：
Y. Wang;KK Morella;J. Ripperger;CF Lai;DP Gearing;GH Fey;SP Campos;H. Baumann
中科院分区：
文献类型：
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作者：
Y. Wang;KK Morella;J. Ripperger;CF Lai;DP Gearing;GH Fey;SP Campos;H. Baumann
为了确定通过盒3基序缺陷型红细胞生成素受体激活信号转导子和转录激活子（STAT）蛋白的特异性，将编码生长激素、白细胞介素-3（IL-3）和IL-4的受体的表达载体与Janus激酶（JAK）和STAT蛋白的表达载体一起瞬时转染到COS-1细胞中。每种受体介导STAT 1和STAT 3的剂量依赖性激活，并且对于IL-3R和GHR，JAK 2增强了该过程。这些数据表明，在JAK/STAT途径的信号转导受体的胞质结构域中的框3基序。将受体与STAT 3组合转染到HepG 2细胞中，通过IL-6反应元件重建了对基因转录的精氨酸依赖性刺激，为STAT 3在控制基因表达中的功能作用提供了证据。
To determine the specificity of signal transducer and activator of transcription (STAT) protein activation by box 3 motif-deficient hematopoietin receptors, expression vectors encoding the receptors for growth hormone, interleukin-3 (IL-3), and IL-4 were transiently transfected into COS-1 cells, together with expression vectors for Janus kinases (JAKs) and STAT proteins. Each receptor mediated a dose-dependent activation of STAT1 and STAT3, and for IL-3R and GHR this process was enhanced by JAK2. The data suggest that a box 3 motif in the cytoplasmic domain of the signal-transducing receptor to the JAK/STAT pathway. Transfection of the receptors, in combination with STAT3, into HepG2 cells reconstituted a cytokine-dependent stimulation of gene transcription through IL-6 response elements, providing evidence for a functional role of STAT3 in controlling gene expression.