The association of lncRNA-HULC polymorphisms with hepatocellular cancer risk and prognosis
The association of lncRNA-HULC polymorphisms with hepatocellular cancer risk and prognosis
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DOI：
10.1016/j.gene.2018.05.096
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发表时间：
2018-09-05
期刊：
影响因子：
3.5
通讯作者：
Yuan, Yuan
中科院分区：
文献类型：
--
作者：
Wang, Ben-gang;Lv, Zhi;Yuan, Yuan
背景：lncRNA HULC基因多态性可能影响癌症的易感性和临床结局。本研究旨在探讨HULC tagSNPs与肝细胞癌风险和预后的关系，以及对lncRNA表达水平的影响。其中，351例HCC患者参与了预后研究。结果：发现启动子区rs 1041279的SNP与肝癌风险增加1.41倍相关（P = 0.032）。在分层分析中，rs 1041279在男性亚组中增加HCC风险的OR更大（P = 0.014，OR = 1.54）。多因素Logistic回归分析显示吸烟-rs 2038540单核苷酸多态性与肝癌风险存在双向交互作用（OR = 4.20）。MDR分析一致表明吸烟-饮酒-rs 2038540 SNP之间的SNP-环境交互作用是预测HCC风险的最佳模型（P = 0.0107）。结论：lncRNA-HULC rs 1041279单核苷酸多态性及其与环境因素的交互作用可增加HCC的风险。1.
Background: Genetic polymorphisms in lncRNA HULC may affect the susceptibility and clinical outcome of cancer. We aimed to investigate the association of HULC tagSNPs with the risk and prognosis of hepatocellular cancer, as well as the influence of the SNPs on lncRNA expression level.Methods: A total of 1338 samples were recruited in the risk study. Among them, 351 HCC patients were involved in the prognosis study. SNP genotyping was performed using RASP method and lncRNA expression was detected by Real-time PCR.Results: We found a promoter SNP, rs1041279, was associated with a 1.41-fold increased HCC risk (P = 0.032). In the stratified analysis, rs1041279 had greater ORs for the increased HCC risk in the male subgroup (P = 0.014, OR = 1.54). Furthermore, multi-logistic regression analysis revealed a two-way interaction effect of smoking-rs2038540 SNP on HCC risk (OR = 4.20). And MDR analysis consistently demonstrated a SNP-environmental interaction among smoking-drinking-rs2038540 SNP as the best model for predicting HCC risk (P = 0.0107). In our study, no significant association was found between HULC SNPs and the overall survival (P > 0.05), and no significant effect was observed of rs1041279 SNP on lncRNA-HULC expression (P > 0.05).Conclusion: lncRNA-HULC rs1041279 SNP and the interaction of rs2038540 SNP with environmental factors could enhance HCC risk. 1.