Targeted Systems Biology Profiling of Tomato Fruit Reveals Coordination of the Yang Cycle and a Distinct Regulation of Ethylene Biosynthesis during Postclimacteric Ripening
Targeted Systems Biology Profiling of Tomato Fruit Reveals Coordination of the Yang Cycle and a Distinct Regulation of Ethylene Biosynthesis during Postclimacteric Ripening
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DOI：
10.1104/pp.112.206086
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发表时间：
2012-11-01
期刊：
影响因子：
7.4
通讯作者：
Geeraerd, Annemie H.
中科院分区：
文献类型：
--
作者：
Van de Poel, Bram;Bulens, Inge;Geeraerd, Annemie H.
更年期果实成熟期间系统1和系统2乙烯生物合成的概念最初在四十年前被描述。尽管人们对果实发育和更年期成熟了解很多，但关于更年期后阶段乙烯生物合成如何调节的信息却很少。一种有针对性的系统生物学方法揭示了番茄（Solanum lycopersicum）乙烯生物合成的新调节机制，当果实达到最大乙烯生产水平时，其特征是乙烯生物合成下降。乙烯生产在 1-氨基环丙烷-1-羧酸氧化酶水平下停止。同时，1-氨基环丙烷-1-羧酸合酶活性增加。阳周期分析表明，阳周期基因以协调方式调控，并在更年期后成熟过程中高表达。与离体果实中的调节相比，跃变后红色番茄植株上的 1-氨基环丙烷-1-羧酸合酶和阳循环基因仅表现出中等调节。用 1-甲基环丙烷和乙烯利处理红色水果表明，乙烯生物合成的关闭机制是发育程序性的，并且仅对乙烯中度敏感。我们提出，成熟果实中系统2的自催化乙烯生物合成的终止可延迟衰老并保存果实直至种子传播。
The concept of system 1 and system 2 ethylene biosynthesis during climacteric fruit ripening was initially described four decades ago. Although much is known about fruit development and climacteric ripening, little information is available about how ethylene biosynthesis is regulated during the postclimacteric phase. A targeted systems biology approach revealed a novel regulatory mechanism of ethylene biosynthesis of tomato (Solanum lycopersicum) when fruit have reached their maximal ethylene production level and which is characterized by a decline in ethylene biosynthesis. Ethylene production is shut down at the level of 1-aminocyclopropane-1-carboxylic acid oxidase. At the same time, 1-aminocyclopropane-1-carboxylic acid synthase activity increases. Analysis of the Yang cycle showed that the Yang cycle genes are regulated in a coordinated way and are highly expressed during postclimacteric ripening. Postclimacteric red tomatoes on the plant showed only a moderate regulation of 1-aminocyclopropane-1-carboxylic acid synthase and Yang cycle genes compared with the regulation in detached fruit. Treatment of red fruit with 1-methylcyclopropane and ethephon revealed that the shut-down mechanism in ethylene biosynthesis is developmentally programmed and only moderately ethylene sensitive. We propose that the termination of autocatalytic ethylene biosynthesis of system 2 in ripe fruit delays senescence and preserves the fruit until seed dispersal.